Edenberg H J, Waqar M A, Huberman J A
Nucleic Acids Res. 1977 Sep;4(9):3083-95. doi: 10.1093/nar/4.9.3083.
We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Replicating SV40 chromosomes isolated from such gradients are capable of continuing DNA synthesis in vitro in the same manner as two crude subnuclear systems we have previously described (4). This indicates that the enzymes and other proteins required for in vitro DNA synthesis are bound to the replicating chromosomes.
我们已部分纯化了处于可在体外持续进行DNA合成形式的猴病毒40（SV40）复制染色体。我们首先从感染SV40的猴细胞中制备一种可溶性DNA合成系统，然后通过极低离子强度的中性蔗糖梯度对各组分进行沉降。从这种梯度中分离出的复制型SV40染色体能够以与我们之前描述的两种粗制亚核系统相同的方式在体外继续进行DNA合成（4）。这表明体外DNA合成所需的酶和其他蛋白质与复制染色体结合在一起。
