在极低盐浓度下提取转录的SV40染色体。
Extraction of transcribing SV40 chromosomes in very low salt.
作者信息
Edenberg H J
出版信息
Nucleic Acids Res. 1980 Feb 11;8(3):573-86. doi: 10.1093/nar/8.3.573.
Abstract
SV40 chromosomes capable of continued RNA synthesis in vitro have been extracted from infected cells in solutions of very low ionic strength (5 mM HEPES, 0.25 mM MgCl2). The RNA made is of high molecular weight, and synthesis is sensitive to alpha-amanitin. More RNA is made than from previously described (high salt-extracted) transcription complexes. Transcribing SV40 chromosomes sediment at a rate intermediate between replicating and mature chromosomes, and have a higher ratio of protein to nucleic acid than either. They retain proteins that are lost during exposure to high salt, and might prove valuable in identifying proteins involved in SV40 transcription.
摘要
能够在体外持续进行RNA合成的SV40染色体已在极低离子强度（5 mM HEPES，0.25 mM MgCl2）的溶液中从受感染细胞中提取出来。合成的RNA具有高分子量，并且合成对α-鹅膏蕈碱敏感。合成的RNA比先前描述的（高盐提取的）转录复合物更多。正在转录的SV40染色体沉降速率介于复制染色体和成熟染色体之间，并且其蛋白质与核酸的比率高于两者中的任何一个。它们保留了在高盐处理过程中丢失的蛋白质，可能对鉴定参与SV40转录的蛋白质很有价值。
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2
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Biochim Biophys Acta. 1973 Dec 7;331(2):283-94. doi: 10.1016/0005-2787(73)90441-3.
3
Animal DNA-dependent RNA polymerases. 4. Studies on inhibition by rifamycin derivatives.动物DNA依赖性RNA聚合酶。4. 利福霉素衍生物抑制作用的研究。
Eur J Biochem. 1972 Jul 13;28(2):291-300. doi: 10.1111/j.1432-1033.1972.tb01913.x.
4
Sarkosyl activation of RNA polymerase activity in mitotic mouse cells.Sarkosyl对有丝分裂小鼠细胞中RNA聚合酶活性的激活作用。
FEBS Lett. 1974 Aug 30;44(3):330-3. doi: 10.1016/0014-5793(74)81170-1.
5
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Virology. 1974 Sep;61(1):11-21. doi: 10.1016/0042-6822(74)90237-2.
6
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