Pirenne J, D'Silva M, Degiovanni G, Jacquet N, Dunn D L
Department of Surgery, University of Liège, CHU Sart Tilman, Belgium.
Transpl Int. 1993;6(5):258-62. doi: 10.1007/BF00336024.
The small bowel (SB), an organ replete with lymphocytes, may provoke graft-versus-host disease (GVHD) after transplantation (Tx). Since tumor necrosis factor (TNF) has been suspected of mediating the tissue lesions of GVHD, we sought to determine whether TNF could be detected in the serum of rats undergoing GVHD after SBTx or lymphocyte transfer. For this purpose, postoperative serum TNF activity was determined in Lewis x Brown Norway (LBNF1) hybrid rats suffering from GVHD after undergoing transplantation of an entire (group 1; n = 8) or a segmental (group 2; n = 4) Lew SB, or after i.p. injection with lethal doses (500 x 10(6)) of Lew lymphocytes (group 3; n = 3). Control LBNF1 received i.p. small doses (50 x 10(6)) of Lew lymphocytes (group 4; n = 4). Serum TNF activity was assessed using the WEHI bioassay. In rats with acute and lethal GVHD after entire SBTx (group 1) or injection with large doses of lymphocytes (group 3), TNF activity gradually increased and reached high levels by the time the rats were agonal. In segmental SBTx rats (group 2), GVHD was less severe than in entire SBTx rats. Similarly, the increase in TNF activity was less intense and only transient since it had returned to control levels by the time the rats had completely recovered from GVHD. In control rats primed with small doses of lymphocytes (group 4), GVHD did not occur and no increase in TNF activity was detected.(ABSTRACT TRUNCATED AT 250 WORDS)
小肠（SB）是一个富含淋巴细胞的器官，移植（Tx）后可能引发移植物抗宿主病（GVHD）。由于怀疑肿瘤坏死因子（TNF）介导GVHD的组织损伤，我们试图确定在小肠移植（SBTx）或淋巴细胞转移后发生GVHD的大鼠血清中是否能检测到TNF。为此，我们测定了Lewis×Brown Norway（LBNF1）杂交大鼠术后血清TNF活性，这些大鼠在接受完整的（第1组；n = 8）或节段性的（第2组；n = 4）Lewis小肠移植后，或经腹腔注射致死剂量（500×10⁶）的Lewis淋巴细胞（第3组；n = 3）后发生了GVHD。对照组LBNF1大鼠经腹腔注射小剂量（50×10⁶）的Lewis淋巴细胞（第4组；n = 4）。使用WEHI生物测定法评估血清TNF活性。在接受完整SBTx（第1组）或注射大剂量淋巴细胞（第3组）后发生急性致死性GVHD的大鼠中，TNF活性逐渐升高，在大鼠濒死时达到高水平。在节段性SBTx大鼠（第2组）中，GVHD比完整SBTx大鼠轻。同样，TNF活性的升高不那么强烈，只是短暂的，因为在大鼠从GVHD完全恢复时，其已恢复到对照水平。在经小剂量淋巴细胞预处理的对照大鼠（第4组）中，未发生GVHD，也未检测到TNF活性增加。（摘要截断于250字）
