硫酸铵和 MALDI 源内裂解：肽测序的绝佳组合。
Ammonium sulfate and MALDI in-source decay: a winning combination for sequencing peptides.
机构信息
Cellular Neurobiology, NIDA IRP, NIH, Baltimore, Maryland 21224, USA.
出版信息
Anal Chem. 2009 Dec 1;81(23):9585-9. doi: 10.1021/ac901518a.
Abstract
In previous papers, we highlighted the role of ammonium sulfate in increasing peptide fragmentation by in-source decay (ISD). The current work systematically investigated effects of matrix assisted laser desorption ionization (MALDI) extraction delay, peptide amino acid composition, matrix, and ammonium sulfate concentration on peptide ISD fragmentation. The data confirmed that ammonium sulfate increased peptides signal-to-noise ratio as well as their in-source fragmentation, resulting in complete sequence coverage regardless of the amino acid composition. This method is easy, inexpensive, and generates the peptides sequence instantly.
摘要
在之前的论文中，我们强调了硫酸铵在通过源内降解（ISD）增加肽片段化中的作用。本工作系统地研究了基质辅助激光解吸电离（MALDI）提取延迟、肽氨基酸组成、基质和硫酸铵浓度对肽 ISD 片段化的影响。数据证实，硫酸铵增加了肽的信噪比及其源内片段化，从而无论氨基酸组成如何，都能实现完整的序列覆盖。该方法简单、廉价，并能立即生成肽序列。
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