利用脂质成像突出大鼠脑组织中的解剖亚结构。
Highlighting anatomical sub-structures in rat brain tissue using lipid imaging.
作者信息
Delvolve Alice M, Colsch Benoit, Woods Amina S
机构信息
Cellular Neurobiology, NIDA IRP, NIH, 333 Cassell Drive, Room 1120, Baltimore, MD, 21224, USA.
出版信息
Anal Methods. 2011 Aug 1;3(8):1729-1736. doi: 10.1039/C1AY05107E.
Abstract
Cell membranes are made up of a mixture of glycerolipids, sphingolipids, gangliosides and cholesterol. Lipids play important roles in a cell's life. However many of their functions have still to be discovered. In the present work, we describe an efficient, easy and rapid methodology to accurately localize phosphatidylcholines and sphingomyelins from a single coronal rat brain section in the cerebrum area. Matrix assisted laser desorption/ionization (MALDI) mass spectrometry was used to profile and image lipids. The best resolved structure was 25-50 μm in the hippocampus.
摘要
细胞膜由甘油脂质、鞘脂、神经节苷脂和胆固醇混合组成。脂质在细胞生命中发挥着重要作用。然而，它们的许多功能仍有待发现。在本研究中，我们描述了一种高效、简便且快速的方法，用于在大鼠大脑冠状切片的大脑区域中准确地定位磷脂酰胆碱和鞘磷脂。采用基质辅助激光解吸/电离（MALDI）质谱对脂质进行分析和成像。在海马体中分辨率最佳的结构为25 - 50μm。
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