糖皮质激素对大鼠垂体促肾上腺皮质激素信使核糖核酸活性水平的影响。
Glucocorticoid effect on the level of corticotropin messenger RNA activity in rat pituitary.
作者信息
Nakanishi S, Kita T, Taii S, Imura H, Numa S
出版信息
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3283-6. doi: 10.1073/pnas.74.8.3283.
Abstract
In an attempt to understand the molecular mechanism underlying the depressive effect of glucocorticoids on corticotropin production, the level of corticotropin messenger RNA activity in rat pituitaries was measured with the use of the cell-free protein-synthesizing system derived from wheat germ. The large translation product of corticotropin messenger RNA was identified and quantitated by indirect immunoprecipitation with antibody against corticotropin. The level of corticotropin messenger RNA activity was increased 3- to 6-fold by adrenalectomy. Dexamethasone administration to adrenalectomized rats resulted in a marked suppression of corticotropin messenger RNA activity. Cortisol and corticosterone also exhibited a suppressive effect but were less effective than dexamethasone. In contrast, nonglucocorticoids such as progesterone and aldosterone had no suppressive effect. These results indicate that at least part of the glucocorticoid effect on corticotropin production in the pituitary is exerted at the pretranslational level.
摘要
为了了解糖皮质激素对促肾上腺皮质激素产生的抑制作用背后的分子机制，利用源自小麦胚芽的无细胞蛋白质合成系统测量了大鼠垂体中促肾上腺皮质激素信使核糖核酸（mRNA）的活性水平。通过用抗促肾上腺皮质激素抗体进行间接免疫沉淀来鉴定和定量促肾上腺皮质激素mRNA的大翻译产物。肾上腺切除术后，促肾上腺皮质激素mRNA的活性水平增加了3至6倍。给肾上腺切除的大鼠注射地塞米松导致促肾上腺皮质激素mRNA活性明显受到抑制。皮质醇和皮质酮也表现出抑制作用，但效果不如地塞米松。相比之下，孕酮和醛固酮等非糖皮质激素没有抑制作用。这些结果表明，糖皮质激素对垂体中促肾上腺皮质激素产生的影响至少部分是在翻译前水平发挥作用的。
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