Tomihata K, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606, Japan.
Tissue Eng. 1996 Winter;2(4):307-13. doi: 10.1089/ten.1996.2.307.
Carbodiimides were employed for cross-linking of gelatin through amide bond formation to avoid any foreign bond incorporation into the cross-linked gelatin molecules. Cross-linking of gelatin was performed not in aqueous solution but in the form of a film under a heterogenous condition. Ethanol-water mixtures were used as the reaction medium to prevent dissolution of gelatin films. The optimal ethanol concentration in the ethanol-water mixtures was around 80 vol%, and the water content of the cross-linked gelatin film attained after swelling with water at 25 degrees C was as low as 55 wt%. A water-soluble carbodiimide (l-ethyl-3[3-dimethylaminoprophyl] carbodiimide) was more effective for gelatin cross-linking than a water-insoluble carbodiimide (1,3-dicyclohexyl carbodiimide). The optimal temperature for cross-linking with the water-soluble carbodiimide ranged between 15 and 25 degrees C. It was concluded that gelatin cross-linking with carbodiimides is as effective as that with glutaraldehyde, which is most widely used despite the toxicity, at least, so far as the water content of cross-linked gelatin film is concerned.
使用碳二亚胺通过形成酰胺键来交联明胶，以避免任何外来键掺入交联的明胶分子中。明胶的交联不是在水溶液中进行，而是在非均相条件下以薄膜形式进行。使用乙醇 - 水混合物作为反应介质以防止明胶膜溶解。乙醇 - 水混合物中的最佳乙醇浓度约为80体积％，在25℃用水溶胀后获得的交联明胶膜的水含量低至55重量％。水溶性碳二亚胺（1-乙基-3 [3-二甲基氨基丙基]碳二亚胺）比水不溶性碳二亚胺（1,3-二环己基碳二亚胺）对明胶交联更有效。用水溶性碳二亚胺交联的最佳温度在15至25℃之间。得出的结论是，就交联明胶膜的水含量而言，用碳二亚胺交联明胶与用戊二醛交联一样有效，戊二醛尽管有毒但至少是目前使用最广泛的。
