Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Phatumwan, Bangkok, Thailand.
J Mater Sci Mater Med. 2013 Mar;24(3):735-44. doi: 10.1007/s10856-012-4830-0. Epub 2012 Dec 8.
The modification of human cancellous bone (hBONE) with silk fibroin/gelatin (SF/G) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccini-mide (NHS) crosslinking was established. The SF/G solutions at a weight ratio of 50/50 and the solution concentrations of 1, 2, and 4 wt % were studied. SF/G sub-matrix was formed on the surface and inside pore structure of hBONE. All hBONE scaffolds modified with SF/G showed smaller pore sizes, less porosity, and slightly lower compressive modulus than unmodified hBONE. SF/G sub-matrix was gradually biodegraded in collagenase solution along 4 days. The hBONE scaffolds modified with SF/G, particularly at 2 and 4 wt % solution concentrations, promoted attachment, proliferation, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSC), comparing to the original hBONE. The highest cell number, ALP activity and calcium production were observed for MSC cultured on the hBONE scaffolds modified with 4 wt % SF/G. The mineralization was also remarkably induced in the cases of modified hBONE scaffolds as observed from the deposited calcium phosphate by EDS. The modification of hBONE with SF/G was, therefore, the promising method to enhance the osteoconductive potential of human bone graft for bone tissue engineering.
采用 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC)/N-羟基琥珀酰亚胺(NHS)交联的方法对人松质骨(hBONE)进行丝素蛋白/明胶(SF/G)修饰。研究了重量比为 50/50 的 SF/G 溶液和浓度为 1%、2%和 4%wt 的溶液。SF/G 亚基质形成在 hBONE 的表面和内部孔隙结构上。所有用 SF/G 修饰的 hBONE 支架的孔径都比未修饰的 hBONE 小，孔隙率更低，压缩模量略低。SF/G 亚基质在胶原酶溶液中沿 4 天逐渐降解。与原始 hBONE 相比，SF/G 浓度为 2%和 4%wt 的溶液修饰的 hBONE 支架更能促进骨髓间充质干细胞(MSC)的黏附、增殖和成骨分化。在培养 MSC 的 hBONE 支架上观察到最高的细胞数量、碱性磷酸酶活性和钙产量。在修饰的 hBONE 支架中也明显诱导了矿化，EDS 分析观察到沉积的磷酸钙。因此，SF/G 修饰 hBONE 是一种增强人骨移植物用于骨组织工程的骨传导潜力的有前途的方法。
