Divisão de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Microbiol Res. 2010 Aug 20;165(6):466-82. doi: 10.1016/j.micres.2009.09.005. Epub 2009 Oct 29.
Little is known about the microbial diversity associated with marine macroorganisms, despite the vital role microorganisms may play in marine ecosystems. The aim of the present study was to investigate the diversity of bacteria and fungi isolated from eight marine invertebrate and one algae samples. Data derived from ARDRA and sequencing analyses allowed the identification of marine-derived microorganisms isolated from those samples. Microbial strains identified up to the genus level revealed 144 distinct ribotypes out of 256 fungal strains and 158 distinct ribotypes out of 181 bacterial strains. Filamentous fungi were distributed among 24 different genera belonging to Ascomycota, Zygomycota and Basidiomycota, some of which had never been reported in the literature as marine invertebrate-inhabiting fungi (Pestalotiopsis, Xylaria, Botrysphaeria and Cunnninghamella). Bacterial isolates were affiliated to 41 different genera, being Bacillus, Ruegeria, Micrococcus, Pseudovibrio and Staphylococcus the most abundant ones. Results revealed an unexpected high microbial diversity associated to the macroorganisms which have been collected and suggested the selection of certain microbial taxonomic groups according to the host. The combined data gathered from this investigation contribute to broaden the knowledge of microbial diversity associated to marine macroorganisms, including as a promising source for the discovery of new natural products.
尽管微生物在海洋生态系统中可能发挥着重要作用，但人们对与海洋大型生物相关的微生物多样性知之甚少。本研究的目的是调查从 8 种海洋无脊椎动物和 1 种藻类样本中分离出的细菌和真菌的多样性。ARDRA 和测序分析的数据允许鉴定从这些样本中分离出的海洋来源的微生物。在 256 株真菌菌株和 181 株细菌菌株中，鉴定到的微生物菌株可分为 144 种不同的核糖体型，24 种不同的丝状真菌分布在子囊菌门、接合菌门和担子菌门的 24 个不同属中，其中一些属从未在文献中报道为海洋无脊椎动物栖息真菌（拟盘多毛孢属、丝核菌属、Botrysphaeria 和 Cuninghamella）。细菌分离物与 41 个不同的属有关，其中芽孢杆菌属、雷尔氏菌属、微球菌属、假单胞菌属和葡萄球菌属最为丰富。结果表明，与已收集的大型生物相关的微生物多样性出人意料地高，并表明根据宿主选择某些微生物分类群。本研究综合数据有助于拓宽与海洋大型生物相关的微生物多样性的知识，包括作为发现新天然产物的有希望的来源。
