Division of Engineering and Department of Orthopaedics, Brown University, Providence, RI 02917, USA.
Biomaterials. 2010 Feb;31(4):706-13. doi: 10.1016/j.biomaterials.2009.09.081. Epub 2009 Oct 30.
Infection of an orthopedic prosthesis is undesirable and causes a decrease in the success rate of an implant. Reducing the adhesion of a broad range of bacteria could be an attractive means to decrease infection and allow for subsequent appropriate tissue integration with the biomaterial surface. In this in vitro study, nanometer sized topographical features of titanium (Ti) surfaces, which have been previously shown to enhance select protein adsorption and subsequent osteoblast (bone-forming cell) functions, were investigated as a means to also reduce bacteria adhesion. This study examined the adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa on conventional Ti, nanorough Ti produced by electron beam evaporation, and nanotubular and nanotextured Ti produced by two different anodization processes. This study found that compared to conventional (nano-smooth) Ti, the nanorough Ti surfaces produced by electron beam evaporation decreased the adherence of all of the aforementioned bacteria the most. The conventional and nanorough Ti surfaces were found to have crystalline TiO(2) while the nanotubular and nanotextured Ti surfaces were found to be amorphous. The surface chemistries were similar for the conventional and nanorough Ti while the anodized Ti surfaces contained fluorine. Therefore, the results of this study in vitro study demonstrated that certain nanometer sized Ti topographies may be useful for reducing bacteria adhesion while promoting bone tissue formation and, thus, should be further studied for improving the efficacy of Ti-based orthopedic implants.
感染骨科假体是不理想的，会降低植入物的成功率。减少广泛细菌的黏附可能是一种有吸引力的方法，可以减少感染，并允许随后与生物材料表面进行适当的组织整合。在这项体外研究中，研究了先前已显示可增强特定蛋白质吸附和随后成骨细胞（骨形成细胞）功能的钛（Ti）表面的纳米级形貌，作为减少细菌黏附的一种手段。这项研究检查了金黄色葡萄球菌、表皮葡萄球菌和铜绿假单胞菌在普通 Ti、电子束蒸发产生的纳米粗糙 Ti、通过两种不同阳极氧化工艺产生的纳米管和纳米纹理 Ti 上的黏附。这项研究发现，与普通（纳米光滑）Ti 相比，电子束蒸发产生的纳米粗糙 Ti 表面最能减少上述所有细菌的黏附。发现常规和纳米粗糙 Ti 表面具有结晶 TiO(2)，而纳米管和纳米纹理 Ti 表面则为非晶态。常规和纳米粗糙 Ti 的表面化学性质相似，而阳极氧化 Ti 表面含有氟。因此，这项体外研究的结果表明，某些纳米级 Ti 形貌可能有助于减少细菌黏附，同时促进骨组织形成，因此应进一步研究以提高基于 Ti 的骨科植入物的疗效。
