Lee Jaekoo, Jo Jaehyun, Wan Jungho, Seo Hanseul, Han Seung-Won, Shin Yoon-Jung, Kim Dong-Hyun
PB Business Department, NVP Healthcare Inc., Suwon 16209, Republic of Korea.
Department of Food Regulatory Science, Korea University, Sejong 30019, Republic of Korea.
Foods. 2024 Jul 22;13(14):2301. doi: 10.3390/foods13142301.
Probiotics restore gut microbial balance, thereby providing health-promoting effects to the host. They have long been suggested for managing intestinal disorders caused by pathogens and for improving gut health. This study evaluated the probiotic properties and anti-pathogenic effects of specific probiotic strains against the intestinal pathogens and . The tested strains- LC27, NK33, NK210, NK46, and NK175-were able to survive harsh conditions simulating gastric and intestinal fluids. These strains exhibited good auto-aggregation abilities (41.8-92.3%) and ideal hydrophobicity (30.9-85.6% and 38.3-96.1% for xylene and chloroform, respectively), along with the ability to co-aggregate with . (40.6-68.2%) and . (38.6-75.2%), indicating significant adhesion levels to Caco-2 cells. Furthermore, these strains' cell-free supernatants (CFSs) demonstrated antimicrobial and antibiofilm activity against . and . . Additionally, these strains inhibited gas production by . through fermentative activity. These findings suggest that the strains tested in this study have potential as novel probiotics to enhance gut health.
益生菌可恢复肠道微生物平衡，从而为宿主提供促进健康的功效。长期以来，人们一直建议使用益生菌来管理由病原体引起的肠道疾病并改善肠道健康。本研究评估了特定益生菌菌株对肠道病原体和的益生菌特性及抗病原体作用。受试菌株——LC27、NK33、NK210、NK46和NK175——能够在模拟胃液和肠液的恶劣条件下存活。这些菌株表现出良好的自聚集能力（41.8 - 92.3%）和理想的疏水性（对二甲苯和氯仿的疏水性分别为30.9 - 85.6%和38.3 - 96.1%），同时具有与和共聚集的能力（分别为40.6 - 68.2%和38.6 - 75.2%），表明对Caco - 2细胞有显著的粘附水平。此外，这些菌株的无细胞上清液（CFSs）对和表现出抗菌和抗生物膜活性。另外，这些菌株通过发酵活性抑制产气。这些发现表明，本研究中测试的菌株有潜力作为新型益生菌来增强肠道健康。
