In Vitro Evaluation of Probiotic Properties and Anti-Pathogenic Effects of and Strains as Potential Probiotics.

Probiotics restore gut microbial balance, thereby providing health-promoting effects to the host. They have long been suggested for managing intestinal disorders caused by pathogens and for improving gut health. This study evaluated the probiotic properties and anti-pathogenic effects of specific probiotic strains against the intestinal pathogens and . The tested strains- LC27, NK33, NK210, NK46, and NK175-were able to survive harsh conditions simulating gastric and intestinal fluids. These strains exhibited good auto-aggregation abilities (41.8-92.3%) and ideal hydrophobicity (30.9-85.6% and 38.3-96.1% for xylene and chloroform, respectively), along with the ability to co-aggregate with . (40.6-68.2%) and . (38.6-75.2%), indicating significant adhesion levels to Caco-2 cells. Furthermore, these strains' cell-free supernatants (CFSs) demonstrated antimicrobial and antibiofilm activity against . and . . Additionally, these strains inhibited gas production by . through fermentative activity. These findings suggest that the strains tested in this study have potential as novel probiotics to enhance gut health.