Araki Hiroyuki
Division of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, Japan.
Biochim Biophys Acta. 2010 Mar;1804(3):520-3. doi: 10.1016/j.bbapap.2009.10.020. Epub 2009 Oct 30.
Cyclin-dependent kinases (CDKs) regulate the progression of the cell cycle in eukaryotes. At the onset of chromosomal DNA replication, CDKs phosphorylate two replication proteins, Sld2 and Sld3, in budding yeast. Phosphorylated Sld2 and Sld3 enhance the formation of complexes with the BRCT (BRCA1 C-terminal)-containing replication protein Dpb11. The formation of these complexes is essential and sufficient for the CDK-dependent activation of the initiation of chromosomal DNA replication. Multiple phosphorylation of Sld2 by CDKs fine-tunes the process of complex formation. Here, we discussed the regulation of the initiation step of chromosomal DNA replication via CDK-dependent phosphorylation.
细胞周期蛋白依赖性激酶（CDKs）调节真核生物中细胞周期的进程。在染色体DNA复制开始时，CDKs在芽殖酵母中磷酸化两种复制蛋白Sld2和Sld3。磷酸化的Sld2和Sld3增强了与含BRCT（BRCA1 C末端）的复制蛋白Dpb11形成复合物的能力。这些复合物的形成对于CDK依赖性的染色体DNA复制起始激活至关重要且足够。CDKs对Sld2的多次磷酸化微调了复合物形成的过程。在这里，我们讨论了通过CDK依赖性磷酸化对染色体DNA复制起始步骤的调控。
