Department of Microbial Genetics, National Institute of Genetics, Research Organization of Information and Systems, Japan.
Curr Opin Cell Biol. 2010 Dec;22(6):766-71. doi: 10.1016/j.ceb.2010.07.015. Epub 2010 Aug 20.
Cyclin-dependent kinase (CDK) is essential for the initiation of chromosomal DNA replication. CDK phosphorylates two yeast replication proteins, Sld2 and Sld3, both of which bind to another replication protein, Dpb11 when phosphorylated. These interactions are essential and are the minimal requirements for CDK activation of chromosomal DNA replication. This review discusses how these phosphorylation-dependent interactions initiate DNA replication through the formation of the pre-loading complex (pre-LC) and its interaction with phosphorylated Sld3 on replication origins. These steps are further regulated by multisite phosphorylation of Sld2. Sld3, on the other hand, must be turned over to reassociate with origins. Pol ɛ functions as a component of the pre-LC as well as a replicative DNA polymerase at replication forks.
细胞周期蛋白依赖性激酶（CDK）对于启动染色体 DNA 复制是必不可少的。CDK 磷酸化两个酵母复制蛋白，Sld2 和 Sld3，这两个蛋白在磷酸化后都与另一个复制蛋白 Dpb11 结合。这些相互作用是必需的，也是 CDK 激活染色体 DNA 复制的最低要求。本综述讨论了这些磷酸化依赖性相互作用如何通过形成预加载复合物（pre-LC）及其与复制起点磷酸化 Sld3 的相互作用来启动 DNA 复制。这些步骤进一步受到 Sld2 的多位点磷酸化的调节。另一方面，Sld3 必须翻转以重新与起点结合。Pol ɛ 作为 pre-LC 的一个组成部分以及复制叉处的复制 DNA 聚合酶发挥作用。
