Reynolds J A, Tanford C, Stone W L
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3796-9. doi: 10.1073/pnas.74.9.3796.
Cooperative interaction of L-alpha-didecanoyl phosphatidylcholine with the AI polypeptide from human serum high density lipoprotein results in a complex particle containing 2 molecules of AI and 190 molecules of the lipid. Binding occurs two orders of magnitude above the critical micelle concentration of the lipid and requires the simultaneous presence of low levels of single-chain amphiphiles. The present investigation and previous studies from this laboratory suggest that AI contains "pockets" capable of interacting with a fixed hydrophobic volume and that amphiphilic head groups do not affect the binding capacity.
L-α-二月桂酰磷脂酰胆碱与人血清高密度脂蛋白中的AI多肽的协同相互作用产生了一种复合颗粒,该颗粒包含2个AI分子和190个脂质分子。结合发生在脂质临界胶束浓度以上两个数量级,并且需要同时存在低水平的单链两亲物。本研究以及本实验室之前的研究表明,AI含有能够与固定疏水体积相互作用的“口袋”,并且两亲性头部基团不影响结合能力。