Faculty of Mathematics and Physics, Charles University, Ke Karlovu 3, 12116, Prague, Czech Republic.
J Fluoresc. 2010 Jan;20(1):343-52. doi: 10.1007/s10895-009-0563-y. Epub 2009 Oct 31.
Fluorescence spectral properties of calcofluor (a popular stain used to visualize cell walls of bacteria, yeast and fungi) has been studied. The analysis of calcofluor fluorescence emission spectra measured in a wide range of solvents (including media containing chitin), and in yeast cell suspensions has revealed that the solvatochromic properties of calcofluor ensue essentially from the by solvent-solute hydrogen bonding, or from the hydrogen bonding to cell wall polysaccharides with an eventual contribution of calcofluor aggregation at the cell surface. Preliminary data suggest that calcofluor emission spectra can be employed as a practical marker of variations in the quality of yeast cell wall.
研究了钙荧光素（一种常用于观察细菌、酵母和真菌细胞壁的流行染色剂）的荧光光谱特性。分析了在广泛的溶剂（包括含几丁质的培养基）中以及在酵母细胞悬浮液中测量的钙荧光素荧光发射光谱，结果表明钙荧光素的溶剂化变色性质主要源于溶剂-溶质氢键，或者是与细胞壁多糖的氢键，最终在细胞表面发生钙荧光素聚集。初步数据表明，钙荧光素的发射光谱可以作为酵母细胞壁质量变化的实用标志物。
