Vilardell Felip, Iacobuzio-Donahue Christine A
Department of Pathology, GI/Liver Division, Johns Hopkins Medical Institutions, The Sol Goldman Pancreatic Cancer Research Center, Baltimore, MD, USA.
Methods Mol Biol. 2010;576:279-92. doi: 10.1007/978-1-59745-545-9_14.
High levels of RNases present in the normal pancreas and the abundance of desmoplastic stroma of most pancreatic cancers have traditionally caused difficulty in the extraction of high-quality RNA and gene expression profiling from pancreatic tissues. However, a variety of innovative strategies have made it possible to successfully perform a molecular analysis of pancreatic cancer, and the expression profiles that have been generated have provided tremendous insight into the nature of this aggressive disease. Here, we describe some of these techniques.
正常胰腺中存在的高水平核糖核酸酶以及大多数胰腺癌中大量的促结缔组织增生性基质，传统上给从胰腺组织中提取高质量RNA和进行基因表达谱分析带来了困难。然而，各种创新策略已使成功开展胰腺癌的分子分析成为可能，所生成的表达谱为深入了解这种侵袭性疾病的本质提供了巨大帮助。在此，我们描述其中一些技术。
