Foye Adam, Febbo Phillip G
Departments of Medicine and Molecular Genetics and Microbiology, Duke Institute for Genome Science and Policy, Duke University, Durham, NC, USA.
Methods Mol Biol. 2010;576:293-326. doi: 10.1007/978-1-59745-545-9_15.
Gene profiling and expression analysis using microarrays have made a significant impact on our biological understanding of prostate cancer. The procedures for generating high-quality expression data from prostate cancer cell lines and tumors are not trivial. However, during the past 9 years, methods by which to process samples for gene profiling have been developed. In this chapter, techniques to process prostate cancer specimens either en bloc (macrodissection) or using laser capture microdissection are presented in detail along with extensive technical notes. Although we focus on prostate cancer and discuss the specific methods utilized in our lab, the processes discussed are generalizable to other tumors and amenable to the substitution of alternative instruments and/or commercially available kits.
使用微阵列进行基因谱分析和表达分析对我们在生物学上理解前列腺癌产生了重大影响。从前列腺癌细胞系和肿瘤中生成高质量表达数据的程序并非易事。然而，在过去9年里，已经开发出了用于基因谱分析的样本处理方法。在本章中，将详细介绍对前列腺癌标本进行整体（宏观解剖）或使用激光捕获显微切割处理的技术以及大量技术说明。尽管我们专注于前列腺癌并讨论我们实验室使用的具体方法，但所讨论的过程可推广到其他肿瘤，并且适用于替代仪器和/或市售试剂盒的替换。
