Rubin M A
Department of Pathology, Urology, and the Comprehensive Cancer and Geriatrics Center of the University of Michigan, Ann Arbor, MI 48109, USA.
J Pathol. 2001 Sep;195(1):80-6. doi: 10.1002/path.892.
One difficulty in studying epithelial tumors has been the inability to isolate pure samples for DNA and RNA analysis. Prostate cancer, with its infiltrative nature, is particularly challenging. The Combination of several new technologies should help overcome these hurdles. Laser capture microdissection uses a laser beam to achieve transfer of pure cell populations for isolation of DNA, RNA, and protein. High-throughput analysis of these samples can be achieved by using cDNA expression microarrays. High-density tissue microarrays should allow for validation of differentially expressed genes. This review will concentrate on the application of laser capture microdissection, cDNA microarrays, and tissue microarrays in the area of prostate cancer research.
研究上皮性肿瘤的一个困难在于无法分离出用于DNA和RNA分析的纯样本。前列腺癌具有浸润性，尤其具有挑战性。几种新技术的结合应有助于克服这些障碍。激光捕获显微切割技术利用激光束实现纯细胞群体的转移，以分离DNA、RNA和蛋白质。通过使用cDNA表达微阵列可以实现对这些样本的高通量分析。高密度组织微阵列应能对差异表达基因进行验证。本综述将集中探讨激光捕获显微切割技术、cDNA微阵列和组织微阵列在前列腺癌研究领域的应用。
