Laboratory of Microbiology, Federal Institute of Education, Science, and Technology of Rio de Janeiro (IFRJ), Rio de Janeiro, RJ, Brazil.
Foodborne Pathog Dis. 2010 Mar;7(3):243-7. doi: 10.1089/fpd.2009.0333.
In the present study, 31 coliform strains were isolated from salad, cheese, and meat products sold in commercial establishments in Rio de Janeiro city, and were tested for antibiotic resistance and antimicrobial substance production. Thirteen strains (41.9%) were resistant to at least one antibiotic tested, among which one presented resistance to nine different antibiotics. Two strains (6.4%) exhibited inhibitory activity against the indicator strains, Escherichia coli LMIFRJ and Salmonella enterica I. The antimicrobial substances that they produced were sensitive to proteolytic enzymes, suggesting that they might be bacteriocins. The producer strains were identified as Klebsiella ozaenae and Raoultella terrigena. Although they had similar spectrums of action, the bacteriocins were shown to be different. Both of them were able to inhibit E. coli, Klebsiella, Enterobacter, and Salmonella strains, including antibiotic-resistant ones. Our results suggest that these bacteriocins, named klebicin K and raoultellin L, could have potential use against some foodborne pathogens.
在本研究中，从里约热内卢市商业机构销售的沙拉、奶酪和肉类产品中分离出 31 株大肠菌群，并对其进行了抗生素耐药性和抗菌物质产生的检测。13 株（41.9%）至少对一种测试的抗生素具有耐药性，其中一株对 9 种不同的抗生素具有耐药性。2 株（6.4%）对指示菌株大肠杆菌 LMIFRJ 和肠炎沙门氏菌 I 表现出抑制活性。它们产生的抗菌物质对蛋白水解酶敏感，表明它们可能是细菌素。产生菌被鉴定为奥克萨娜氏克雷伯菌和拉乌尔菌。尽管它们的作用谱相似，但细菌素是不同的。它们都能够抑制包括抗生素耐药菌在内的大肠杆菌、克雷伯菌、肠杆菌和沙门氏菌。我们的结果表明，这两种细菌素，分别命名为 klebicin K 和 raoultellin L，可能对一些食源性病原体具有潜在的应用价值。
