Laboratoire de Physicochimie des Matériaux, IPEST, BP51, La Marsa 2070, Tunisia.
J Food Sci. 2009 Sep;74(7):M364-71. doi: 10.1111/j.1750-3841.2009.01277.x.
GC-FID and GC-MS analysis of essential oils of Juniperus phoenicea resulted in the identification of 30 compounds, representing more than 98% of the total composition. alpha-pinene (55.7% and 80.7%), delta-3-carene (10.7% and 4.5%), and gamma-cadinene (2.9% and 5.1%) were the main components, respectively, in leaves and berries essential oil. Extracts of J. phoenicea were obtained by different extraction solvents: methanol, ethanol, ethyl acetate, and dichloromethane and evaluated composition for polyphenols (gallic acid equivalent 52 to 217 g/kg), tannins (catechin equivalent 6.5 to 60.2 g/kg), antocyanins (cyanidin equivalent 84 to 373 mg/kg), and flavonoids (quercetin equivalent 6.4 to 29.3 g/kg). The samples (essential oils and extracts) were subjected to a screening for their antioxidant activity by using DPPH and ABTS assays; antimicrobial activity was tested with 6 bacteria (3 Gram-positive and 3 Gram-negative), 1 yeast, and 2 fungi. The strongest antioxidant activity was obtained by the methanolic extract (IC(50)= 6.5 +/- 0.3 mg/L). Flavonoids are likely to contribute to the antifungal activity against Saccharomyces cerevisiae. Correlations were studied between chemical composition and antioxidant and antimicrobial activities.
GC-FID 和 GC-MS 分析结果表明，杜松的挥发油由 30 种化合物组成，占总成分的 98%以上。α-蒎烯（55.7%和 80.7%）、δ-3-蒈烯（10.7%和 4.5%）和γ-杜松烯（2.9%和 5.1%）分别是叶油和浆果油的主要成分。采用甲醇、乙醇、乙酸乙酯和二氯甲烷等不同提取溶剂提取杜松提取物，评价其多酚（没食子酸当量 52 至 217 毫克/千克）、单宁（儿茶素当量 6.5 至 60.2 毫克/千克）、花青素（花青素当量 84 至 373 毫克/千克）和类黄酮（槲皮素当量 6.4 至 29.3 毫克/千克）的组成。用 DPPH 和 ABTS 法对样品（挥发油和提取物）进行抗氧化活性筛选；用 6 种细菌（3 种革兰氏阳性菌和 3 种革兰氏阴性菌）、1 种酵母和 2 种真菌测试抗菌活性。结果表明，甲醇提取物的抗氧化活性最强（IC50=6.5±0.3 毫克/升）。类黄酮可能对抑制酿酒酵母具有抗真菌活性。研究了化学成分与抗氧化和抗菌活性之间的相关性。
