Department of Pharmacology, M. M. College of Pharmacy, M. M. University, Mullana, Ambala, Haryana, India.
Diabetes Res Clin Pract. 2010 Jan;87(1):38-43. doi: 10.1016/j.diabres.2009.10.002. Epub 2009 Nov 5.
The study was undertaken to assess the protective effect of Spirulina fusiformis extract against Rosiglitazone induced osteoporosis and pharmacodynamic effects of Rosiglitazone with Spirulina in treating hyperglycemia and hyperlipidemia of insulin resistance rat.
For this aim, 30 Wistar albino rats were equally divided into five groups as control (C), diabetes mellitus (DM), diabetes mellitus+Rosiglitazone (DM+R), diabetes mellitus+Spirulina (DM+S), and diabetes mellitus+Rosiglitazone+Spirulina (DM+R+S). Serum glucose, triglyceride, HDL, LDL and insulin concentrations were estimated by routine standard methods in blood samples collected on 21th day. Integrity of the bone surface was examined by scanning electronic microscopy, and bone strength was measured by micro-hardness test on 45th day.
A significant decrease in total bone mineral density was observed in group DM+R rats (p<0.05). The number and depth of resorptive pits on surface of the bone in Rosiglitazone treated rats improved clearly with Spirulina administration. The intactness and integrity of the bone surface as well as the bone strength improved due to the high content of calcium and phosphorous in Spirulina. Besides, chromium and gamma-linoleic acid in Spirulina helped to decrease the fasting serum glucose, HDL, LDL and triglycerides levels in insulin resistance rats.
These findings suggest that combination therapy of Rosiglitazone with Spirulina reduced the risk of osteoporosis in insulin resistance rats. Additionally, Spirulina complemented the antihyperglycemic and antilipidemic activity of Rosiglitazone.
本研究旨在评估螺旋藻提取物对罗格列酮诱导的骨质疏松的保护作用，以及罗格列酮与螺旋藻联合治疗胰岛素抵抗大鼠高血糖和高血脂的药效学作用。
为此目的，将 30 只 Wistar 白化大鼠等分为五组，分别为对照组（C）、糖尿病组（DM）、糖尿病+罗格列酮组（DM+R）、糖尿病+螺旋藻组（DM+S）和糖尿病+罗格列酮+螺旋藻组（DM+R+S）。在第 21 天采集血样，用常规标准方法测定血清葡萄糖、甘油三酯、HDL、LDL 和胰岛素浓度。第 45 天，用扫描电子显微镜检查骨表面完整性，用微硬度试验测定骨强度。
DM+R 组大鼠总骨矿物质密度显著下降（p<0.05）。螺旋藻给药后，骨表面吸收陷窝的数量和深度明显改善。螺旋藻中高含量的钙和磷使骨表面的完整性和骨强度得到改善。此外，螺旋藻中的铬和γ-亚麻酸有助于降低胰岛素抵抗大鼠的空腹血糖、HDL、LDL 和甘油三酯水平。
这些发现表明，罗格列酮与螺旋藻联合治疗可降低胰岛素抵抗大鼠发生骨质疏松的风险。此外，螺旋藻补充了罗格列酮的降血糖和抗血脂作用。
