Quispe-Mauricio Angel, Callacondo David, Rojas José, Zavala David, Posso Margarita, Vaisberg Abraham
Sociedad Científica de San Fernando, Universidad Nacional Major de San Marcos, Lima, Perú.
Rev Gastroenterol Peru. 2009 Jul-Sep;29(3):239-46.
The plants have been used as drugs for centuries. However, limited research has been done on its great potential as sources of new therapeutic agents. The purpose of this study was to evaluate Physalis peruviana cytotoxic activity on cell lines HT-29, PC-3, K-562 and VERO.
The HT-29 cell lines, PC-3, K-562 and VERO, were exposed to four concentrations of P. peruviana ethanolic leave and stem extracts, also at different concentrations of cisplatin and 5-fluorouracil (5-FU), which were used as positive controls. We found rates of growth within 48 hours, then we determined the inhibitory concentration 50 (IC50) using linear regression analysis and the index of selectivity of each sample.
The P. peruviana ethanolic leave and stem extracts showed cytotoxic activity. The IC50 in g/mL in leaves and stems were, 0.35 (r =-0.95 p <0.025) and 0.37 (r =- 0.90 p <0.05 ) for HT-29; 0.87 (r =-0.98 p <0.01) and 1.01 (r =-0.95 p <0.025) for PC-3; 0.02 (r =-0.98 p <0.01) and 0.03 (r =-0.98 p <0.01) for K-562; 4.9 (r =-0.95 p <0.025) and 6.2 (r =-0.98 p <0.01) for VERO. The IC50 for antineoplastic were: for cisplatin: 4.2 (r =-0.96 p <0.025), 10.3 (r =-0.97 p <0.025), 0.15 (r =-0.98 p = 0.01) and 1.1 (r =- 0.98 p = 0.01); for 5-FU: 2.3 (r =-0.97 p <0.025), 17.9 (r =-0.95 p <0.025), 0.15 (r =-0.98 p = 0.01) and 1.1 (r =-0.94 p = 0.05) for HT-29, PC-3, K562 and VERO respectively. The leaves and stems extracts selectivity index were between 5.6 and 245 for tumor cell lines evaluated, by contrast, cisplatin and 5-FU, only showed values between 0.11 and 7.3.
The P. peruviana leaves and steams ethanolic extracts were more cytotoxic than cisplatin and 5 FU, on the lines HT-29, PC-3 and K562. Furthermore the P. peruviana cytotoxic effects were less than cisplatin and 5-FU for VERO control cells lines.
几个世纪以来，植物一直被用作药物。然而，对于其作为新型治疗剂来源的巨大潜力，人们的研究还很有限。本研究的目的是评估灯笼果对HT - 29、PC - 3、K - 562和VERO细胞系的细胞毒性活性。
将HT - 29细胞系、PC - 3、K - 562和VERO细胞暴露于四种浓度的灯笼果乙醇叶提取物和茎提取物，同时也暴露于不同浓度的顺铂和5 - 氟尿嘧啶（5 - FU），它们被用作阳性对照。我们在48小时内测定了细胞生长速率，然后使用线性回归分析确定了半数抑制浓度（IC50）以及每个样品的选择性指数。
灯笼果乙醇叶提取物和茎提取物显示出细胞毒性活性。对于HT - 29细胞系，叶提取物和茎提取物的IC50（以μg/mL计）分别为0.35（r = - 0.95，p < 0.025）和0.37（r = - 0.90，p < 0.05）；对于PC - 3细胞系，分别为0.87（r = - 0.98，p < 0.01）和1.01（r = - 0.95，p < 0.025）；对于K - 562细胞系，分别为0.02（r = - 0.98，p < 0.01）和0.03（r = - 0.98，p < 0.01）；对于VERO细胞系，分别为4.9（r = - 0.95，p < 0.025）和6.2（r = - 0.98，p < 0.01）。抗肿瘤药物的IC50分别为：顺铂，对于HT - 29、PC - 3、K562和VERO细胞系，分别为4.2（r = - 0.96，p < 0.025）、10.3（r = - 0.97，p < 0.025）、0.15（r = - 0.98，p = 0.01）和1.1（r = - 0.98，p = 0.01）；5 - FU，对于HT - 29、PC - 3、K562和VERO细胞系，分别为2.3（r = - 0.97，p < 0.025）、17.9（r = - 0.95，p < 0.025）、0.15（r = - 0.98，p = 0.01）和1.1（r = - 0.94，p = 0.05）。在所评估的肿瘤细胞系中，叶提取物和茎提取物的选择性指数在5.6至245之间，相比之下，顺铂和5 - FU的选择性指数仅在0.11至7.3之间。
在HT - 29、PC - 3和K562细胞系中，灯笼果叶和茎的乙醇提取物比顺铂和5 - FU具有更强的细胞毒性。此外，对于VERO对照细胞系，灯笼果的细胞毒性作用小于顺铂和5 - FU。
