Franco Luis A, Matiz Germán E, Calle Jairo, Pinzón Roberto, Ospina Luis F
Departamento de Farmacia, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Cartagena, Cartagena, Colombia.
Biomedica. 2007 Mar;27(1):110-5. Epub 2007 May 31.
Cape gooseberry calyces (Physalis peruviana) have been used in folk medicine for their medicinal properties including anticancer, antimycobacterial, antipyretic, diuretic, immunomodulatory and antiinflammatory properties.
The antiinflammatory effect was evaluated for extracts and fractions obtained from Physalis peruviana calyces in a mice model of acute inflammation. The fractions responsible for antiinflammatory activity were extracted for possible identification.
The Physalis peruviana calyces were extracted by percolation with organic solvents. The primary hydroalcoholic fraction was purified by column chromatography. The antiinflammatory effect of extracts and fractions was evaluated using the 12-O-tetradecanoylphorbol-13-acetate-induced mouse model of ear edema.
Thirty-eight secondary fractions were obtained by column chromatography of primary hydroalcoholic fraction. Six fractions, evaluated in 12-O-tetradecanoylphorbol-13-acetate-induced inflammation assay, showed significant antiinflammatory activity (p<0.05). The major fraction, Pp-D28-LF, showed a significant dose-dependent response at doses over 250 microg/ear.
The antiinflammatory activity attributed to Physalis peruviana calyces was confirmed and validated its use in folk medicine. Fractions responsible for the antiinflammatory action were identified and seem promising for phytomedicinal development. Further studies are needed to isolate and identify the active constituents of these fractions as well as to ascertain the mechanisms involved in the antiinflammatory effect.
灯笼椒萼片（酸浆）因其具有抗癌、抗分枝杆菌、解热、利尿、免疫调节和抗炎等药用特性，已被用于民间医学。
在急性炎症小鼠模型中评估从灯笼椒萼片中获得的提取物和馏分的抗炎作用。提取具有抗炎活性的馏分以便进行可能的鉴定。
用有机溶剂渗漉法提取灯笼椒萼片。通过柱色谱法纯化初级水醇馏分。使用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯诱导的小鼠耳水肿模型评估提取物和馏分的抗炎作用。
通过对初级水醇馏分进行柱色谱法获得了38个二级馏分。在12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯诱导的炎症试验中评估的6个馏分显示出显著的抗炎活性（p<0.05）。主要馏分Pp - D28 - LF在剂量超过250微克/耳时显示出显著的剂量依赖性反应。
证实了灯笼椒萼片具有抗炎活性，并验证了其在民间医学中的应用。确定了具有抗炎作用的馏分，这些馏分在植物医学开发方面似乎很有前景。需要进一步研究分离和鉴定这些馏分的活性成分以及确定抗炎作用所涉及的机制。
