通过原子转移自由基聚合（ATRP）用温敏性 PNIPAM 对羟基磷灰石纳米粒子进行表面改性。

Surface modification of hydroxyapatite nanoparticles with thermal-responsive PNIPAM by ATRP.

机构信息

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.

出版信息

Macromol Biosci. 2009 Dec 8;9(12):1237-46. doi: 10.1002/mabi.200900256.

DOI:10.1002/mabi.200900256

PMID:19899073

Abstract

Hydroxyapatite (HA) nanoparticles grafted by poly(N-isopropylacrylamide) (PNIPAM) brushes (PNIPAM-g-HA) have been synthesized by the surface-initiated atom transfer radical polymerization (ATRP) of N-isopropylacrylamide (NIPAM). The surface grafting amount of PNIPAM ranges from 15.5% to 46.4%. PNIPAM-g-HA has been characterized by FT-IR spectroscopy, thermal gravimetric analysis, X-ray diffraction, and scanning electron microscopy (SEM). The UV transmittance spectra and the particle size analysis of PNIPAM-g-HA in aqueous solution demonstrates that the PNIPAM-g-HA possess reversible thermal stimuli responsive properties. An in vitro bioactivity assessment indicates that PNIPAM-g-HA can induce the mineralization of Ca(2+) and HPO(4) (2-) and possesses an excellent bioactivity. The cell culture results show that the cells adhered to the surface of PNIPAM-g-HA grow better than on HA, and the area of the cells on the surface of PNIPAM-g-HA is much greater than for HA, which proves that the PNIPAM-g-HA has a better biocompatibility than HA.

摘要

通过表面引发原子转移自由基聚合（ATRP）接枝聚 N-异丙基丙烯酰胺（PNIPAM）制备了羟基磷灰石（HA）纳米粒子接枝的 PNIPAM 刷（PNIPAM-g-HA）。PNIPAM 的表面接枝量范围为 15.5%至 46.4%。通过傅里叶变换红外光谱（FT-IR）、热重分析（TGA）、X 射线衍射（XRD）和扫描电子显微镜（SEM）对 PNIPAM-g-HA 进行了表征。PNIPAM-g-HA 在水溶液中的紫外透光光谱和粒径分析表明，PNIPAM-g-HA 具有可逆的热刺激响应特性。体外生物活性评估表明，PNIPAM-g-HA 可以诱导 Ca(2+)和 HPO(4)（2-）的矿化，具有优异的生物活性。细胞培养结果表明，与 HA 相比，细胞在 PNIPAM-g-HA 表面的黏附性更好，且在 PNIPAM-g-HA 表面的细胞面积比在 HA 上的更大，这证明了 PNIPAM-g-HA 比 HA 具有更好的生物相容性。