直接对映选择性 HPLC 监测脂肪酶催化的 flurbiprofen 动力学拆分。

Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen.

机构信息

Australian Centre for Research On Separation Science (ACROSS), School of Chemistry, University of Tasmania, Hobart, Tasmania, Australia.

出版信息

Chirality. 2010 Jun;22(6):597-603. doi: 10.1002/chir.20798.

DOI:10.1002/chir.20798

PMID:19899153

Abstract

The solvent versatility of Chiralpak IB, a 3,5-dimethylphenylcarbamate derivative of cellulose-based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase-catalysis and in mean time as diluent to dissolve the "difficult to dissolve" enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization.

摘要

手性固定相 3,5-二甲基苯基氨基甲酸酯纤维素衍生物 Chiralpak IB 的溶剂多功能性在非标准 HPLC 有机溶剂中脂肪酶催化动力学拆分氟比洛芬的直接对映选择性 HPLC 监测中得到了证明。非标准 HPLC 有机溶剂既被用作脂肪酶催化的反应介质，同时也被用作稀释剂来溶解“难溶解”的酶底物（酸），并作为洗脱剂，在一次运行中无需进一步衍生化，即可同时对两种底物和产物进行对映选择性 HPLC 基线分离。