Buxant F, Fayt I, Noel J C
Department of Gynecology, Erasme Hospital, Free University of Brussels, Brussels, Belgium.
Eur J Gynaecol Oncol. 2009;30(5):500-2.
Paget disease of the nipple is a rare disease characterized by the presence of malignant glandular cells within the squamous epithelium of the nipple. The most common hypothesis to explain the development of Paget's disease is an intraepithelial epidermotropic migration of malignant epithelial cells originating from an underlying intraductal carcinoma. Although the immunohistochemical properties of Paget cells in the nipple have been extensively studied, their proliferating characteristics remain paradoxically poorly studied. In the present study we have investigated the proliferating activity of Paget cells in the nipple by using double stain immunohistochemistry with both Ki-67 (a protein which is expressed in all active parts of the cell cycle) and cytokeratin 7 (a highly sensitive marker of Paget cells). Ten cases of Paget's disease and the associated intraductal carcinomas (n = 10) and/or invasive carcinomas (n = 4) were tested. The mean Ki-67 index was in Paget's disease (26% +/- 10), in intraductal carcinomas (23% +/- 8) and/or in invasive carcinomas (20% +/- 8) (p > 0.05). This is the first report to convincingly demonstrate by specific double stain immunohistochemistry that Paget's disease and underlying intraductal carcinomas share a close proliferating activity.
乳头佩吉特病是一种罕见疾病，其特征是乳头鳞状上皮内存在恶性腺细胞。解释佩吉特病发展的最常见假说是，起源于潜在导管内癌的恶性上皮细胞发生上皮内表皮趋向性迁移。尽管对乳头佩吉特细胞的免疫组化特性已进行了广泛研究，但令人费解的是，其增殖特性的研究仍然很少。在本研究中，我们通过使用Ki-67（一种在细胞周期所有活跃部分均有表达的蛋白质）和细胞角蛋白7（佩吉特细胞的高敏标志物）进行双重染色免疫组化，研究了乳头佩吉特细胞的增殖活性。对10例佩吉特病及相关导管内癌（n = 10）和/或浸润性癌（n = 4）进行了检测。佩吉特病的平均Ki-67指数为（26%±10），导管内癌为（23%±8）和/或浸润性癌为（20%±8）（p>0.05）。这是第一份通过特异性双重染色免疫组化令人信服地证明佩吉特病与潜在导管内癌具有密切增殖活性的报告。
