Departments of Pathology, Erasme Hospital, Free University of Brussels, 808 route de Lennik, 1070, Brussels, Belgium.
Pathol Oncol Res. 2010 Mar;16(1):7-10. doi: 10.1007/s12253-009-9179-4. Epub 2009 Dec 17.
Paget disease of the nipple is a rare disease characterized by the presence of malignant glandular cells within the squamous epithelium of the nipple. The most common hypothesis to explain the development of Paget disease is an intraepithelial epidermotropic migration of malignant epithelial cells originating from an underlying intraductal carcinoma. If the immunohistochemical properties of the Paget cells in the nipple have been extensively studied, their proliferating characteristics remain paradoxically poorly studied. In the present study we have investigated the proliferating activity of Paget cells in the nipple by using double stain immunohistochemistry with both Ki67 (a protein which is expressed in all active parts of the cell cycle) and cytokeratin 7 (a highly sensitive marker of Paget cells). Ten cases of Paget disease and in their associated intraductal carcinomas (n = 10) and/or invasive carcinomas (n = 4) were tested. The mean Ki67 index was in Paget disease (26% +/- 10), in intraductal carcinomas (23% +/- 8) and/or in invasive carcinomas (20% +/- 8) (p > 0,05). This is the first report to convincingly demonstrate by specific double stain immunohistochemistry that Paget disease and underlying intraductal carcinomas share a close proliferating activity.
乳头派杰病是一种罕见疾病，其特征是乳头的鳞状上皮中存在恶性腺细胞。解释派杰病发展的最常见假说为源自于下方的导管内癌的恶性上皮细胞的上皮内表皮移行。虽然乳头派杰细胞的免疫组织化学特性已被广泛研究，但它们的增殖特性仍出人意料地研究不足。在本研究中，我们通过使用双重染色免疫组织化学技术（同时检测 Ki67 和细胞角蛋白 7）来研究乳头派杰细胞的增殖活性。我们共检测了 10 例派杰病及其相关的导管内癌（n = 10）和/或浸润性癌（n = 4）。派杰病的平均 Ki67 指数为 26% +/- 10%，导管内癌为 23% +/- 8%和/或浸润性癌为 20% +/- 8%（p > 0.05）。这是首次通过特异性双重染色免疫组织化学证实派杰病和下方的导管内癌具有密切的增殖活性的报道。
