Barbosa Flávia Leão, Góes Rejane Maira, de Faria-E-Sousa Sidney Júlio, Haddad Antonio
Department of Ophthalmology, Faculty of Medicine of Ribeirão Preto University of São Paulo, Ribeirão Preto, SP, Brazil.
Curr Eye Res. 2009 Aug;34(8):636-45. doi: 10.1080/02713680903007121.
To investigate the proliferative behavior of the corneal and limbal epithelia after debridement on the central region of the rabbit cornea.
After scraping a circular epithelial area, 5 mm in diameter, in the center of the cornea, ([3]) H-thymidine ( ([3]) H-TdR) was injected intravitreally, and the rabbits killed from 1 to 49 days afterward. The cornea, together with the adjacent conjunctiva, was processed for autoradiography.
The regenerating epithelium at the center of the cornea exhibited high frequencies of labeled nuclei when compared to controls. The mitotic indexes for the limbus were comparable in experimental and control eyes. The unique basal stratum of the limbal epithelium exhibited quick proliferation and vertical migration in all eyes. Cells that remained labeled for four weeks or more were observed throughout the corneal epithelium, including its basal stratum, and this did not depend on epithelial damage.
Corneal epithelium wounds are healed by sliding and proliferation of cells surrounding the epithelial gap without any evidence for the participation of the limbal epithelium. Daughter cells labeled with ([3]) H-TdR were visualized in all layers of the corneal epithelium up to 7 weeks after the DNA precursor injection. However, at this long interval, the only labeled cells in the limbus were in the suprabasal layers.
研究兔角膜中央区清创术后角膜和角膜缘上皮的增殖行为。
在角膜中央刮除直径5 mm的圆形上皮区域后，经玻璃体腔内注射[³H]胸腺嘧啶核苷（[³H]TdR），随后在1至49天内处死兔子。将角膜连同相邻的结膜进行放射自显影处理。
与对照组相比，角膜中央的再生上皮显示出高频率的标记细胞核。实验眼和对照眼角膜缘的有丝分裂指数相当。角膜缘上皮独特的基底细胞层在所有眼中均表现出快速增殖和垂直迁移。在整个角膜上皮包括其基底细胞层中均观察到标记持续四周或更长时间的细胞，这与上皮损伤无关。
角膜上皮伤口通过上皮间隙周围细胞的滑动和增殖而愈合，没有任何证据表明角膜缘上皮参与其中。在注射DNA前体后长达7周的时间里，在角膜上皮的所有层中均可见用[³H]TdR标记的子代细胞。然而，在如此长时间段时，角膜缘中唯一标记的细胞位于基底上层。
