Departamento de Oftalmologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 2012 May;45(5):408-10. doi: 10.1590/s0100-879x2012007500023. Epub 2012 Mar 1.
How is the corneal epithelium restored when all of it plus the limbus have been eliminated? This investigation explored the possibility that this may be achieved through the conjunctival epithelium. The corneal epithelium of the right eye of 12 rabbits (Oryctolagus cuniculus) was totally scraped followed by surgical excision of the limbus plus 1.0-1.5 mm of the adjacent conjunctiva. Antibiotics and corticosteroids were applied for 1 week after surgery. Histological and immunohistochemical techniques were used to monitor the events taking place on the eye surface 2 weeks and 1, 3 and 6 months thereafter. Initially, the corneal surface was covered by conjunctival-like epithelium. After 1 month and more prominently at 3 and 6 months an epithelium displaying the morphological features of the cornea and reacting with the AE5 antibody was covering the central region. It is likely that the corneal epithelium originated from undifferentiated cells of the conjunctiva interacting with the corneal stroma.
当全部角膜上皮和角膜缘都被去除时，角膜上皮是如何被修复的？本研究探索了通过结膜上皮来实现这一过程的可能性。通过手术切除兔子（Oryctolagus cuniculus）右眼的全部角膜上皮和 1.0-1.5mm 毗邻的结膜，来造成角膜上皮和角膜缘的完全损伤。手术后 1 周内使用抗生素和皮质类固醇。术后 2 周、1 个月、3 个月和 6 个月时，采用组织学和免疫组织化学技术监测眼表面发生的情况。最初，角膜表面被类似结膜的上皮覆盖。1 个月后，更明显的是在 3 个月和 6 个月时，中央区域覆盖了具有角膜形态特征并与 AE5 抗体反应的上皮。很可能角膜上皮来源于与角膜基质相互作用的结膜未分化细胞。
