Xue Chunyi, Zhang Yun, Zhou Qingfeng, Xu Cong, Li Xiaoming, Cao Yongchang
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
J Vet Diagn Invest. 2009 Nov;21(6):841-3. doi: 10.1177/104063870902100612.
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of Infectious bursal disease virus (IBDV). The RT-LAMP assay used a set of 4 primers to amplify the viral protein 2 gene of IBDV for the detection of IBDV, showing not only high efficiency but also analytic specificity. The data demonstrated that the RT-LAMP assay detected 30 different IBDV isolates, had no cross-reaction with 3 other avian viruses (Infectious bronchitis virus, Newcastle disease virus, and Avian influenza virus), and obtained a 95.45% sensitivity in 22 positive clinical samples in reference to virus isolation. Therefore, this rapid, specific, sensitive, and convenient RT-LAMP assay could be applicable to the identification of IBDV in less-equipped laboratories as well as in the field.
开发了一种逆转录环介导等温扩增（RT-LAMP）检测方法，用于快速鉴定传染性法氏囊病病毒（IBDV）。该RT-LAMP检测方法使用一组4条引物扩增IBDV的病毒蛋白2基因以检测IBDV，不仅显示出高效率，而且具有分析特异性。数据表明，该RT-LAMP检测方法检测了30种不同的IBDV分离株，与其他3种禽病毒（传染性支气管炎病毒、新城疫病毒和禽流感病毒）无交叉反应，相对于病毒分离，在22份阳性临床样本中获得了95.45%的灵敏度。因此，这种快速、特异、灵敏且便捷的RT-LAMP检测方法可适用于设备简陋的实验室以及现场对IBDV的鉴定。
