Spraker Terry R, VerCauteren Kurt C, Gidlewski Tom L, Munger Randy D, Walter W David, Balachandran Aru
USDA/APHIS/Wildlife Services/National Wildlife Research Center, Fort Collins, CO 80521, USA.
J Vet Diagn Invest. 2009 Nov;21(6):868-70. doi: 10.1177/104063870902100618.
To determine if the number of rectal lymphoid follicles decreases with respect to age and sex relative to diagnosis of chronic wasting disease (CWD), rectal biopsies (n = 1,361) were taken from captive Rocky Mountain elk (Cervus elaphus nelsoni) at 4 ranches in the western United States between 2005 and 2008. Rectal tissues were stained with a monoclonal antibody (F99/97.6.1), which selectively stains the abnormal isoform of the prion protein associated with CWD of elk. The number of lymphoid follicles obtained from typical biopsy tissues decreased with the age of the animal. The acceptable number of lymphoid follicles for detection of CWD was not considered to be a problem in elk up to 8.5 years of age, but in elk over 8.5 years of age, the follicle count was considered to be low. Sex of the animal had no effect on the number of lymphoid follicles observed in each age group. Rectal biopsies were an accurate test to diagnose preclinical stages of CWD in elk but may be best suited to elk that are less then 8.5 years of age.
为了确定相对于慢性消耗病（CWD）的诊断，直肠淋巴滤泡的数量是否随年龄和性别而减少，2005年至2008年期间，在美国西部4个牧场对圈养的落基山麋鹿（Cervus elaphus nelsoni）进行了直肠活检（n = 1361）。直肠组织用单克隆抗体（F99/97.6.1）染色，该抗体可选择性地标记与麋鹿CWD相关的朊病毒蛋白异常异构体。从典型活检组织中获得的淋巴滤泡数量随动物年龄的增长而减少。在8.5岁以下的麋鹿中，检测CWD的可接受淋巴滤泡数量不被视为问题，但在8.5岁以上的麋鹿中，滤泡计数被认为较低。动物的性别对各年龄组观察到的淋巴滤泡数量没有影响。直肠活检是诊断麋鹿CWD临床前期阶段的准确检测方法，但可能最适合8.5岁以下的麋鹿。
