State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, People's Republic of China.
Biomed Mater. 2009 Dec;4(6):065004. doi: 10.1088/1748-6041/4/6/065004.
The aim of this study is to improve hippocampal cell adhesion on silicon (Si) surfaces by surface modification via a chemical method. Collagen and chitosan were first immobilized on a Si surface through a bridging layer of (3-aminopropyl) triethoxysilane and 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide. The modified Si surface was then characterized by contact angle measurement, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy and atomic force microscopy. The amounts of protein adsorbed on the bare Si surface and collagen/chitosan-modified Si surface were measured by a modified Coomassie brilliant blue protein assay. The cell adhesion behavior was then assessed by a microscopy. The results showed that after the surface modification with the collagen/chitosan compound the protein adsorption on Si surfaces decreased while the adhesion ability of hippocampal cells increased.
本研究旨在通过化学方法对硅（Si）表面进行表面改性，从而提高海马细胞在 Si 表面的黏附能力。首先通过（3-氨丙基）三乙氧基硅烷和 1-乙基-3-(3-二甲基氨丙基)碳二亚胺的桥连层将胶原和壳聚糖固定在 Si 表面上。然后通过接触角测量、傅里叶变换红外光谱、X 射线光电子能谱和原子力显微镜对修饰后的 Si 表面进行表征。通过改良考马斯亮蓝蛋白法测量裸 Si 表面和胶原/壳聚糖修饰 Si 表面上吸附的蛋白量。然后通过显微镜评估细胞黏附行为。结果表明，经过胶原/壳聚糖复合的表面改性后，Si 表面上的蛋白吸附量减少，而海马细胞的黏附能力增加。
