Tao Caihong, Zhang Junyan, Yang Shengrong
School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
J Nanosci Nanotechnol. 2011 Jun;11(6):5068-74. doi: 10.1166/jnn.2011.4144.
This paper describes a general strategy for grafting protein molecules on silicon surface by using dopamine as adhesive layer. With this method, silicon surface had been successfully modified by BSA monolayer. Fourier transform infrared spectra, X-ray photoelectron spectroscopy, contact angle analysis and atomic force microscopy confirmed the sequential grafting of initiator and protein molecules. Cell adhesion experiments with PC-12 cells showed that the obtained monolayer exhibits good biocompatibility. The corrosion resistance behavior of the polydopamine and BSA modified silicon wafers was investigated by potentiodynamic test, which indicated that the modified surfaces exhibited a better anti-corrosion capability than silicon surface. All these results must be valuable for the application of protein monolayer in biological and biomedical technology.
本文描述了一种利用多巴胺作为粘附层在硅表面接枝蛋白质分子的通用策略。通过这种方法，已成功地用牛血清白蛋白单分子层修饰了硅表面。傅里叶变换红外光谱、X射线光电子能谱、接触角分析和原子力显微镜证实了引发剂和蛋白质分子的顺序接枝。用PC-12细胞进行的细胞粘附实验表明，所获得的单分子层具有良好的生物相容性。通过动电位测试研究了聚多巴胺和牛血清白蛋白修饰的硅片的耐腐蚀行为，结果表明修饰后的表面比硅表面具有更好的抗腐蚀能力。所有这些结果对于蛋白质单分子层在生物和生物医学技术中的应用必定具有重要价值。
