Guil-Guerrero José Luis, Campra Pablo
Madrid Área de Tecnología de Alimentos, Universidad de Almería, 04120 Almería, Spain.
Trop Biomed. 2009 Aug;26(2):149-54.
A chemical-ecology approach has been used to screen plants growing in Guyana Highlands as an indicator of production of biologically active secondary metabolites. Extracts of leaves from 19 species, most of them endemic in this area, and collected at the top of Roraima Tepui (2,723 m) were screened in vitro at different concentrations for their potential cytotoxic activity against three tumour cell lines: HT29 (colon), A549 (lung) and MDA-MB-231 (breast). MTT (tetrazolium blue) colorimetric assay was employed as cytotoxicity test. Extracts of nine species caused less than 30% growth in at least one cell line. From these species, high cytotoxic activity was detected in Casearia sylvestris var. lingua and Ledotamnus sessiliflorus extracts; medium activity was found in Cyathea sp. Two other species, Cyrilla racemiflora and Heliamphora minor showed lower but significant cytotoxicity. Further cytotoxicity-directed fractionation of these extracts would be advisable to isolate and identify the active principles of these plants.
一种化学生态学方法已被用于筛选生长在圭亚那高地的植物，以此作为生物活性次生代谢产物产生的指标。从19种植物的叶子中提取的提取物，其中大多数是该地区特有的，采集于罗赖马山（2723米）顶部，在体外针对三种肿瘤细胞系：HT29（结肠）、A549（肺）和MDA-MB-231（乳腺），以不同浓度筛选其潜在的细胞毒性活性。采用MTT（四唑蓝）比色法作为细胞毒性试验。九种植物的提取物在至少一种细胞系中导致的生长抑制率低于30%。在这些植物中，在舌形桤叶树（Casearia sylvestris var. lingua）和无柄花石南叶柃（Ledotamnus sessiliflorus）提取物中检测到高细胞毒性活性；在某种桫椤（Cyathea sp.）中发现中等活性。另外两种植物，总状 Cyrilla racemiflora和小叶太阳瓶子草（Heliamphora minor）显示出较低但显著的细胞毒性。对这些提取物进行进一步的细胞毒性导向分级分离，以分离和鉴定这些植物的活性成分是可取的。
