Silva A F S, de Andrade J P, Machado K R B, Rocha A B, Apel M A, Sobral M E G, Henriques A T, Zuanazzi J A S
Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-010 Porto Alegre, RS, Brazil.
Phytomedicine. 2008 Oct;15(10):882-5. doi: 10.1016/j.phymed.2007.12.001. Epub 2008 Mar 4.
The dichloromethane and n-butanol extracts obtained from fresh bulbs of Hippeastrum vittatum (Amaryllidaceae), collected in Southern Brazil, were evaluated for their cytotoxic activity in vitro against five human cell lines (HT29 colon adenocarcinoma, H460 non-small cell lung carcinoma, RXF393 renal cell carcinoma, MCF7 breast cancer, and OVCAR3 epithelial ovarian cancer), using the sulphorhodamine B assay. Both extracts showed potential antiproliferative activity. From CH(2)Cl(2) fraction, three alkaloids were isolated: lycorine, vittatine and montanine. The two last compounds were submitted to the antiproliferative assay and the highest level of cytotoxicity was found for the alkaloid montanine.
对采自巴西南部的朱顶红（石蒜科）新鲜鳞茎所获得的二氯甲烷和正丁醇提取物，采用磺酰罗丹明B测定法，评估其对五种人类细胞系（HT29结肠腺癌、H460非小细胞肺癌、RXF393肾细胞癌、MCF7乳腺癌和OVCAR3上皮性卵巢癌）的体外细胞毒性活性。两种提取物均显示出潜在的抗增殖活性。从二氯甲烷馏分中分离出三种生物碱：石蒜碱、朱顶红碱和山地明碱。后两种化合物进行了抗增殖试验，发现生物碱山地明碱的细胞毒性水平最高。
