Kumara T K, Abu Hassan A, Che Salmah M R, Bhupinder S
School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.
Trop Biomed. 2009 Aug;26(2):200-5.
Larvae of the Synthesiomyia nudiseta (Wulp) were collected from a decomposed human corpse at the Department of Forensic Medicine, Penang Hospital. A colony of this species was established and the eggs were collected for rearing. The developmental times, rearing temperatures, and relative humidity were recorded twice daily from the time the eggs collected until adult emergence. An average of 5 larvae were randomly collected from the rearings twice daily, warm-water killed and preserved in Kahle's solution. The larval instar stages were determined by observing the number of posterior spiracular slits and the length of the preserved larvae were measured. When the larval life cycle was completed, the accumulated developmental times were calculated. A total of 8 replicates were carried out. The temperature of the rearing room was 28.5+/-1.5 degrees Celcius while the relative humidity was within 67-85%. The total developmental time for S. nudiseta was 322+/-19 hours (13.4+/-0.8 days).
从槟城医院法医学部的一具腐烂人体尸体上采集了裸芒综蝇（Synthesiomyia nudiseta (Wulp)）的幼虫。建立了该物种的一个种群，并收集了卵用于饲养。从收集卵到成虫羽化期间，每天记录两次发育时间、饲养温度和相对湿度。每天从饲养群体中随机收集平均5只幼虫，用温水杀死并保存在卡尔氏溶液中。通过观察后气门裂的数量来确定幼虫龄期，并测量保存的幼虫长度。当幼虫生命周期完成后，计算累积发育时间。总共进行了8次重复实验。饲养室温度为28.5±1.5摄氏度，相对湿度在67 - 85%之间。裸芒综蝇的总发育时间为322±19小时（13.4±0.8天）。
