固定化粒细胞集落刺激因子在细胞毒性骨髓抑制中刺激粒细胞生成活性的机制。

Mechanisms of granulocytopoiesis-stimulating activity of immobilized granulocytic colony-stimulating factor in cytostatic myelosuppression.

作者信息

Dygai A M, Vereshchagin E I, Zhdanov V V, Zyuz'kov G N, Ermakova N N, Madonov P G, Miroshnichenko L A, Minakova M Yu, Simanina E V, Stavrova L A, Udut E V, Firsova T V, Khrichkova T Yu

机构信息

Institute of Pharmacology, Tomsk Research Center, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia.

出版信息

Bull Exp Biol Med. 2009 Jul;148(1):49-53. doi: 10.1007/s10517-009-0626-8.

DOI:10.1007/s10517-009-0626-8

PMID:19902095

Abstract

The hemostimulatory effects of granulocytic CSF, immobilized on polyethyleneglycol using radiation synthesis nanotechnology, were studied on the model of cyclophosphamide-induced myelosuppression. Immobilization of granulocytic CSF led to stimulation of granulomonocytopoiesis by increasing functional activity of granulomonocytic precursors and secretion of humoral factors by elements of hemopoiesis-inducing microenvironment, and due to more intensive formation of hemopoietic islets. The granulocytopoiesis-stimulating effect of immobilized granulocytic CSF was comparable to the effect of standard nonconjugated granulocytic CSF. Specific activity of immobilized granulocytic CSF in oral treatment was demonstrated.

摘要

采用辐射合成纳米技术将粒细胞集落刺激因子（granulocytic CSF）固定于聚乙二醇上，在环磷酰胺诱导的骨髓抑制模型上研究了其促止血作用。粒细胞集落刺激因子的固定化通过提高粒单核细胞前体的功能活性以及造血诱导微环境成分分泌体液因子，导致粒单核细胞生成受到刺激，并且由于造血岛的形成更加密集。固定化粒细胞集落刺激因子的粒细胞生成刺激作用与标准非共轭粒细胞集落刺激因子的作用相当。证明了固定化粒细胞集落刺激因子在口服治疗中的比活性。