College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, People's Republic of China.
Biol Trace Elem Res. 2010 Oct;137(1):69-78. doi: 10.1007/s12011-009-8560-1. Epub 2009 Nov 10.
Lead acetate (300 mg/L) and/or cadmium chloride (50 mg/L) were administered as drinking water to Sprague-Dawley rats for 8 weeks to investigate the possible combined effects of these metals on the damage in renal cortex mitochondria. Increased malonaldehyde levels due to exposure to these metals were detected by colorimetric method, which demonstrated the lipid peroxidation in the renal cortex. Ultrastructural observations and real-time quantitative PCR analyses were performed on kidney cortex pieces to identify the mitochondrial damage and quantify the relative expression levels of cytochrome oxidase subunits (COX-I/II/III), respectively. The most striking ultrastructural modifications involved distortion of mitochondrial cristae and an unusual arrangement, which were more evident when the mixture was ingested. There were significant differences in the expression levels of COX-I, II, and III between the control group and the exposed groups, whereas those in the (lead+cadmium) group were all significantly lower than that in the lead or cadmium group. In conclusion, there was an obvious synergistic oxidative damage effect of lead combined with cadmium on rat kidney cortex mitochondria, which increased defects in mitochondrial oxidative metabolism.
给 Sprague-Dawley 大鼠喂食醋酸铅（300mg/L）和/或氯化镉（50mg/L）作为饮用水，持续 8 周，以研究这些金属对肾皮质线粒体损伤的可能联合影响。通过比色法检测到这些金属暴露导致的丙二醛水平升高，表明肾皮质发生了脂质过氧化。对肾皮质组织进行超微结构观察和实时定量 PCR 分析，分别鉴定线粒体损伤和量化细胞色素氧化酶亚基（COX-I/II/III）的相对表达水平。最显著的超微结构改变涉及线粒体嵴的扭曲和异常排列，当摄入混合物时更为明显。与对照组相比，暴露组 COX-I、II 和 III 的表达水平均有显著差异，而（铅+镉）组的表达水平均明显低于铅组或镉组。总之，铅与镉联合对大鼠肾皮质线粒体具有明显的协同氧化损伤作用，增加了线粒体氧化代谢的缺陷。
