Wang Lin, Chen Dawei, Wang Heng, Liu Zongping
College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, People's Republic of China.
Biol Trace Elem Res. 2009 Summer;129(1-3):190-9. doi: 10.1007/s12011-008-8288-3. Epub 2008 Dec 19.
Lead acetate (300 mg/L) and/or cadmium chloride (50 mg/L) were administered as drinking water of Sprague-Dawley rats for 8 weeks to investigate the possible combined effect of these metals on the expression of metallothionein (MT) in kidneys. Immunohistochemical staining and real-time quantitative polymerase chain reaction analyses were performed on kidney samples to identify the distribution of MT and to quantify the relative expression levels of MT-1 and MT-2 gene, respectively. There is no significant difference in distribution of the intensity, amount of MT immunostaining, and expression level of MT-1 and MT-2 gene in the kidneys between the lead group and control group (P > 0.05), whereas those in the cadmium group and (Pb + Cd) group were significantly higher than that in the control group (P < 0.01). Compared to the (Pb + Cd) group with the lead or cadmium group, the expression level of MT-1 and MT-2 gene increased significantly (P < 0.01). In summary, there was an obvious synergistic effect of lead combined with cadmium on the expression of metallothionein in the kidney of rats.
将醋酸铅（300毫克/升）和/或氯化镉（50毫克/升）作为饮用水给予Sprague-Dawley大鼠8周，以研究这些金属对大鼠肾脏中金属硫蛋白（MT）表达的可能联合作用。对肾脏样本进行免疫组织化学染色和实时定量聚合酶链反应分析，以分别确定MT的分布并量化MT-1和MT-2基因的相对表达水平。铅组与对照组相比，肾脏中MT免疫染色的强度、数量以及MT-1和MT-2基因的表达水平分布无显著差异（P>0.05），而镉组和（铅+镉）组的上述指标均显著高于对照组（P<0.01）。与铅组或镉组相比，（铅+镉）组中MT-1和MT-2基因的表达水平显著升高（P<0.01）。综上所述，铅和镉联合对大鼠肾脏中金属硫蛋白的表达有明显的协同作用。
