Institute of Biology, University of Southern Denmark, Odense, Campusvej 55, 5230 Odense M, Denmark.
Anal Bioanal Chem. 2010 Jan;396(2):631-40. doi: 10.1007/s00216-009-3262-2. Epub 2009 Nov 10.
To evaluate the interaction between 17beta-trenbolone (TB) and 17alpha-ethinylestradiol (EE2), male eelpout, Zoarces viviparus, was exposed for 21 days (April to May 2008) to 5 ng l(-1) EE2 and 5 or 20 ng l(-1) TB, separately or in combination in a flow-through SW system. The effects on hepatosomatic (HSI) and gonadosomatic index (GSI), plasma vitellogenin (Vtg) concentration, gonadal histology, hepatic and testicular Vtg mRNA and estrogen receptor (ERalpha) mRNA expression were investigated. No effects on HSI were observed. A significant decrease was observed in the GSI of all males exposed to EE2 (<0.7%) when compared to controls (1.4%). Histological alterations and immature stages were observed in the testis of all exposed males; however, males exposed to EE2 were the most affected. Increased tubule number and proportionally decreased tubule diameter were observed in the testis of all EE2 groups. No effects in Vtg mRNA expression were observed in the testis; however, a significant decrease in testis ERalpha mRNA was observed in males exposed to 20 ng l(-1) TB. The groups exposed to EE2 showed a significant increase in plasma Vtg (>300-fold), hepatic Vtg mRNA (>450-fold), and ERalpha mRNA (>100-fold) when compared to controls. This study shows that lower concentrations of 17beta-trenbolone are unable to counteract the EE2 estrogenic effects when the exposure is simultaneous.
为了评估 17β-群勃龙（TB）和 17α-乙炔基雌二醇（EE2）之间的相互作用，雄性欧鲶，Zoarces viviparus，在 21 天（2008 年 4 月至 5 月）内分别暴露于 5ng/L 的 EE2 和 5 或 20ng/L 的 TB 中，或在流动 SW 系统中联合暴露。研究了对肝体比（HSI）和性腺体比（GSI）、血浆卵黄蛋白原（Vtg）浓度、性腺组织学、肝和睾丸 Vtg mRNA 和雌激素受体（ERα）mRNA 表达的影响。未观察到 HSI 的变化。与对照组（1.4%）相比，所有暴露于 EE2 的雄性的 GSI 均显著降低（<0.7%）。所有暴露于 EE2 的雄性的睾丸均观察到组织学改变和未成熟阶段；然而，暴露于 EE2 的雄性受影响最大。所有 EE2 组的睾丸小管数量增加，小管直径比例减小。在睾丸中未观察到 Vtg mRNA 表达的变化；然而，暴露于 20ng/L TB 的雄性睾丸 ERα mRNA 显著降低。与对照组相比，暴露于 EE2 的组的血浆 Vtg 显著增加（>300 倍），肝 Vtg mRNA （>450 倍）和 ERα mRNA （>100 倍）增加。本研究表明，当暴露同时进行时，较低浓度的 17β-群勃龙无法抵消 EE2 的雌激素作用。
