Institute of Biology, University of Southern Denmark, Odense, Campusvej 55, Odense M, Denmark.
Mar Environ Res. 2010;69 Suppl:S67-9. doi: 10.1016/j.marenvres.2009.09.009. Epub 2009 Oct 1.
To evaluate the interaction between 17β-trenbolone (TB) and 17α-ethinylestradiol (EE2) in relevant environmental concentrations, male eelpout Zoarces viviparus were exposed in a flow-through seawater-system for 21 days to 5 ng l⁻¹ EE2, 5 ng l⁻¹ or 20 ng l⁻¹ TB or to combinations of both compounds. The effects on hepatosomatic index (HSI), gonadosomatic index (GSI) and gonadal histology were studied. No significant effects on HSI were observed in any treatment; in contrast, decreased GSI was observed in males exposed to EE2 alone or in combination with TB compared to controls (p<0.05). The histology revealed that the males were in the beginning of spermatogenesis. Males from the control group and some from the TB groups showed tubules with cysts containing spermatogonia, spermatocytes and spermatids; however, some testes of males exposed to TB showed slight to moderate interstitial fibrosis. Nevertheless, the most severely affected were males exposed to EE2 showing marked interstitial fibrosis, necrosis of germinal cells and reduced number of spermatocytes and spermatogonia in the cyst. Likewise, increased tubule number and proportionally decreased tubule diameter were observed in the testis of all EE2 exposed groups (p<0.05). Finally, a similar tubule number was observed in males exposed to EE2+20 ng l⁻¹ TB compared to control (p>0.05). This study shows that EE2 dramatically disrupts the spermatogenesis and low doses of 17β-trenbolone are unable to effectively counteract the morphological effects of EE2.
为了评估 17β- trenbolone（TB）和 17α-乙炔基雌二醇（EE2）在相关环境浓度下的相互作用，雄性欧拟鲈 Zoarces viviparus 在流动海水系统中暴露 21 天，暴露于 5ng/L EE2、5ng/L 或 20ng/L TB 或两种化合物的组合中。研究了对肝体比（HSI）、性腺体比（GSI）和性腺组织学的影响。在任何处理中均未观察到 HSI 的显著影响；相反，与对照组相比，单独暴露于 EE2 或与 TB 联合暴露的雄性的 GSI 降低（p<0.05）。组织学显示雄性处于精子发生的早期。来自对照组和一些 TB 组的雄性具有包含精原细胞、精母细胞和精子细胞的小管中的囊肿；然而，一些暴露于 TB 的睾丸显示出轻微到中度的间质纤维化。然而，受 EE2 暴露影响最严重的是雄性，其表现为明显的间质纤维化、生殖细胞坏死以及囊中的精母细胞和精原细胞数量减少。同样，在所有 EE2 暴露组的睾丸中观察到管数增加和管直径比例降低（p<0.05）。最后，与对照组相比，暴露于 EE2+20ng/L TB 的雄性观察到相似的管数（p>0.05）。本研究表明，EE2 严重破坏精子发生，而低剂量的 17β-trenbolone 无法有效抵消 EE2 的形态效应。
