PETROBRAS-CENPES-Centro de Pesquisas e Desenvolvimento, Gerência de Energias Renováveis, Rio de Janeiro, RJ, Brazil.
J Ind Microbiol Biotechnol. 2010 Feb;37(2):151-8. doi: 10.1007/s10295-009-0656-2. Epub 2009 Nov 10.
The objective of this work is to investigate the utilization of two abundant agricultural residues in Brazil for the production and application of cellulolytic enzymes. Different materials obtained after pretreatment of sugarcane bagasse, as well as pure synthetic substrates, were considered for cellulase production by Penicillium funiculosum. The best results for FPase (354 U L(-1)) and beta-glucosidase (1,835 U L(-1)) production were observed when sugarcane bagasse partially delignified cellulignin (PDC) was used. The crude extract obtained from PDC fermentation was then partially characterized. Optimal temperatures for cellulase action ranged from 52 to 58 degrees C and pH values of around 4.9 contributed to maximum enzyme activity. At 37 degrees C, the cellulases were highly stable, losing less than 15% of their initial activity after 23 h of incubation. There was no detection of proteases in the P. funiculosum extract, but other hydrolases, such as endoxylanases, were identified (147 U L(-1)). Finally, when compared to commercial preparations, the cellulolytic complex from P. funiculosum showed more well-balanced amounts of beta-glucosidase, endo- and exoglucanase, resulting in the desired performance in the presence of a lignocellulosic material. Cellulases from this filamentous fungus had a higher glucose production rate (470 mg L(-1) h(-1)) when incubated with corn cob than with Celluclast, GC 220 and Spezyme (312, 454 and 400 mg L(-1) h(-1), respectively).
本工作旨在研究利用巴西两种丰富的农业废弃物生产和应用纤维素酶。使用长枝木霉（Penicillium funiculosum）生产纤维素酶时，考虑了不同预处理后的甘蔗渣材料以及纯合成底物。当使用部分脱木质素纤维素木质素（PDC）时，FPase（354 U L(-1)）和β-葡萄糖苷酶（1,835 U L(-1)）的产量达到最佳。然后对来自 PDC 发酵的粗提取物进行了部分表征。纤维素酶作用的最佳温度范围为 52-58°C，pH 值约为 4.9 有助于最大酶活性。在 37°C 下，纤维素酶高度稳定，孵育 23 小时后初始活性损失小于 15%。在长枝木霉提取物中未检测到蛋白酶，但鉴定出其他水解酶，如内切木聚糖酶（147 U L(-1)）。最后，与商业制剂相比，长枝木霉的纤维素酶复合体系具有更平衡的β-葡萄糖苷酶、内切和外切葡聚糖酶含量，在存在木质纤维素材料时表现出所需的性能。当用玉米芯孵育时，丝状真菌的纤维素酶的葡萄糖生成速率（470 mg L(-1) h(-1)）高于用 Celluclast、GC 220 和 Spezyme（分别为 312、454 和 400 mg L(-1) h(-1)）。
