Luong Thi Thu Huong, Poeaim Supattra, Tangthirasunun Narumon
Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang (KMITL), Bangkok, Thailand.
Mycobiology. 2023 Aug 30;51(4):239-245. doi: 10.1080/12298093.2023.2247221. eCollection 2023.
Xylanase has been applied in various sectors, such as biomass conversion, paper, pulp, textiles, and pharmaceutical industries. This study aimed to isolate and screen potential xylanase-producing fungi from the soil of Suphan Buri Province, Thailand. Fifteen fungi were isolated, and their xylanase activities were tested by the qualitative method. The result showed that isolate SP3, SP10 and SP15 gave high xylanase activity with potency index (PI) of 2.32, 2.01 and 1.82, respectively. These fungi were selected for the xylanase quantitative test, isolate SP10 performed the highest xylanase activity with 0.535 U/mL. Through molecular methods using the gene, isolate SP10 was identified as . The xylanase characteristics from SP10 were determined, including the xylanase isoforms and the optimum pH and temperature. The xylanase isoforms on SDS-PAGE indicated that SP10 produced two xylanases (45 and 54 kDa). Moreover, its xylanase worked optimally at pH 6 and 55 °C while reaching 61% activity at 65 °C. These results proposed SP10 as a good candidate for industrial uses, especially in poultry feed and pulp industries, to improve yield and economic efficiency under slightly acidic and high-temperature conditions.
木聚糖酶已应用于各个领域，如生物质转化、造纸、制浆、纺织和制药行业。本研究旨在从泰国素攀武里府的土壤中分离和筛选潜在的产木聚糖酶真菌。分离出15株真菌，并通过定性方法检测它们的木聚糖酶活性。结果表明，分离株SP3、SP10和SP15具有较高的木聚糖酶活性，效价指数（PI）分别为2.32、2.01和1.82。这些真菌被选作木聚糖酶定量测试，分离株SP10的木聚糖酶活性最高，为0.535 U/mL。通过使用该基因的分子方法，分离株SP10被鉴定为。测定了SP10的木聚糖酶特性，包括木聚糖酶同工型以及最适pH和温度。SDS-PAGE上的木聚糖酶同工型表明，SP10产生两种木聚糖酶（45和54 kDa）。此外，其木聚糖酶在pH 6和55°C时活性最佳，在65°C时活性达到61%。这些结果表明，SP10是工业应用的良好候选者，特别是在家禽饲料和制浆工业中，在微酸性和高温条件下可提高产量和经济效益。
