Div. of Textile and Clothing, University of California, Davis, CA 95616, USA.
Biotechnol Prog. 2010 Mar-Apr;26(2):352-60. doi: 10.1002/btpr.346.
A novel marine bacterium strain effectively produced prodiginine type pigments. These colorants could dye wool, silk and synthetic fabrics such as polyester and polyacrylic and also show antibacterial properties against Escherichia coli and Staphylococcus aureus bacteria on the dyed products. Methyl nitrosoguanidine was used as a mutation agent to increase the genetic diversity and the production yield of the bacteria of the family of Vibrio gazogenes. The analysis of the mutated samples showed that two new main colorants as well as three previously found ones were produced. Liquid chromatography electro spray ionization mass spectrometry (LC-ESI-MS) and nuclear magnetic resonance (NMR) spectroscopic techniques were used to elucidate the structures of the newly produced colorants. Mass measurements revealed that the colorants C1, C2, C3, C4 have molecular masses of 321, 323, 351, and 295 Da. One unstable colorant C5 with molecular mass of 309 Da was detected as well. The mutated bacteria strains increased the yield of pigment production by about 81% and produced prodigiosin in 97% purity. The antibiotic activities of pure colorants are discussed as well. Based on their bio-activity and excellent dyeing capabilities, these colorants could be employed in cosmetic and textile industries.
一种新型海洋细菌菌株能有效产生灵菌红素类色素。这些着色剂可以给羊毛、丝绸和合成纤维（如聚酯和聚丙烯腈纤维）染色，并且对染色产品上的大肠杆菌和金黄色葡萄球菌具有抗菌性能。亚甲基硝胍被用作诱变剂，以增加弧菌科细菌的遗传多样性和生产产量。对突变样本的分析表明，产生了两种新的主要着色剂以及三种之前发现的着色剂。采用液相色谱电喷雾电离质谱（LC-ESI-MS）和核磁共振（NMR）光谱技术阐明了新产生的着色剂的结构。质量测量表明，着色剂 C1、C2、C3、C4 的分子量分别为 321、323、351 和 295 Da。还检测到一种不稳定的着色剂 C5，分子量为 309 Da。突变菌增加了色素产量约 81%，并以 97%的纯度产生灵菌红素。还讨论了纯着色剂的抗生素活性。基于其生物活性和出色的染色能力，这些着色剂可以用于化妆品和纺织工业。
