de Rond Tristan, Stow Parker, Eigl Ian, Johnson Rebecca E, Chan Leanne Jade G, Goyal Garima, Baidoo Edward E K, Hillson Nathan J, Petzold Christopher J, Sarpong Richmond, Keasling Jay D
Department of Chemistry, University of California, Berkeley, California, USA.
Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California, USA.
Nat Chem Biol. 2017 Nov;13(11):1155-1157. doi: 10.1038/nchembio.2471. Epub 2017 Sep 11.
Prodiginines, which are tripyrrole alkaloids displaying a wide array of bioactivities, occur as linear and cyclic congeners. Identification of an unclustered biosynthetic gene led to the discovery of the enzyme responsible for catalyzing the regiospecific C-H activation and cyclization of prodigiosin to cycloprodigiosin in Pseudoalteromonas rubra. This enzyme is related to alkylglycerol monooxygenase and unrelated to RedG, the Rieske oxygenase that produces cyclized prodiginines in Streptomyces, implying convergent evolution.
灵菌红素是一类具有多种生物活性的三吡咯生物碱，有线性和环状类似物。通过鉴定一个未聚类的生物合成基因，发现了负责催化红色假交替单胞菌中灵菌红素区域特异性碳氢键活化和环化生成环灵菌红素的酶。该酶与烷基甘油单加氧酶相关，与链霉菌中产生环化灵菌红素的铁硫簇加氧酶RedG无关，这意味着趋同进化。
