Elandaloussi Laurence, Carrasco Noèlia, Furones Dolores, Roque Ana
Institut de Recerca i Tecnologia Agroalimentaries (IRTA)-Sant Caries de la Rapita, Ctra. Poble nou, Km 5,5, 43540 Sant Caries de la Rapita, Spain.
Dis Aquat Organ. 2009 Sep 23;86(2):135-42. doi: 10.3354/dao02107.
The phylogenetic relationship of Perkinsus olseni originating from the Ebro Delta, Spain, to other Perkinsus spp. was investigated using the nontranscribed spacer (NTS) region and the internal transcribed spacer (ITS) region (including ITS1, 5.8S and ITS2) of the ribosomal DNA sequences. These 2 molecular markers (NTS and ITS) were sequenced from prezoosporangia of Perkinsus sp. originating from Manila clam Ruditapes philippinarum from the Ebro Delta. The sequence of the 5.8S ITS region of the ribosomal RNA gene was 100% similar to that of P. olseni. Higher genetic variability was found for the NTS sequence, with 80.7 to 81.8% similarity to P. olseni. The NTS sequence of a P. olseni isolate previously detected in R. decussatus from the same area was also obtained and showed 81% identity with our isolate. Evidence obtained from phylogenetic analysis of the 5.8S ITS and NTS aligned sequences appears to indicate that P. olseni strains group together according to their host rather than their geographic origins within a well-resolved P. olseni clade.
利用核糖体DNA序列的非转录间隔区（NTS）和内部转录间隔区（ITS，包括ITS1、5.8S和ITS2），研究了源自西班牙埃布罗三角洲的奥尔森派金虫与其他派金虫物种之间的系统发育关系。对源自埃布罗三角洲菲律宾蛤仔的派金虫的前游动孢子囊进行了这两个分子标记（NTS和ITS）的测序。核糖体RNA基因的5.8S ITS区域序列与奥尔森派金虫的序列100%相似。发现NTS序列具有更高的遗传变异性，与奥尔森派金虫的相似性为80.7%至81.8%。还获得了先前在同一地区的四角蛤蜊中检测到的奥尔森派金虫分离株的NTS序列，与我们的分离株显示出81%的同一性。从5.8S ITS和NTS比对序列的系统发育分析中获得的证据似乎表明，在一个解析良好的奥尔森派金虫进化枝中，奥尔森派金虫菌株是根据其宿主而非地理来源聚集在一起的。
