Wu Shao-Qiang, Wang Cai-Xia, Lin Xiang-Mei, Wang Zong-Xiang, Li Xiao-Fei, Liu Jian, Deng Jun-Hua, Qiu Song-Yin
Institute of Animal and Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, PR China.
Dis Aquat Organ. 2011 Aug 29;96(1):55-60. doi: 10.3354/dao02353.
The prevalence of Perkinsus sp. infection in Manila clam Ruditapes philippinarum was investigated in the coastal areas of east China. Thirteen groups of clams were collected from 5 sites: Dandong and Qingdao Bays (Yellow Sea), Weifang Bay (Bohai Sea), and Ningbo and Fuzhou Bays (East China Sea). The clams were tested for perkinsosis infection using Ray's fluid thioglycollate medium culture assay. Perkinsus sp. was found in samples from all 5 sites from May 2008 to May 2009. Infection prevalence ranged from 43.75 to 95.83%, and was significantly higher in October than in May. The only 3 uninfected groups of clams were collected from Weifang Bay, the site farthest from the ocean. There was no difference in the prevalence of infection among the remaining 4 sites. The conserved internal transcribed spacer regions of the ribosomal RNA gene complex in each of the Perkinsus sp. isolates were amplified by PCR. The resulting amplicons were sequenced and phylogenetically analyzed. All the Perkinsus isolates were identified as Perkinsus olseni.
在中国东部沿海地区调查了菲律宾蛤仔（Ruditapes philippinarum）中奥尔森派琴虫（Perkinsus olseni）感染的流行情况。从5个地点采集了13组蛤仔：丹东湾和青岛湾（黄海）、潍坊湾（渤海）以及宁波湾和福州湾（东海）。使用雷氏硫乙醇酸盐培养基培养法对蛤仔进行派琴虫病感染检测。在2008年5月至2009年5月期间，在所有5个地点的样本中均发现了奥尔森派琴虫。感染率在43.75%至95.83%之间，10月份的感染率显著高于5月份。仅有的3组未感染蛤仔是从距离海洋最远的潍坊湾采集的。其余4个地点的感染率没有差异。通过PCR扩增了每个奥尔森派琴虫分离株核糖体RNA基因复合体的保守内部转录间隔区。对所得扩增产物进行测序并进行系统发育分析。所有派琴虫分离株均被鉴定为奥尔森派琴虫。
