Huchin-Mian Juan Pablo, Briones-Fourzán Patricia, Simá-Alvarez Raúl, Cruz-Quintana Yanis, Pérez-Vega Juan Antonio, Lozano-Alvarez Enrique, Pascual-Jiménez Cristina, Rodríguez-Canul Rossanna
Laboratorio de Inmunología y Biologia Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-Unidad Mérida, Km 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán 97310, Mexico.
Dis Aquat Organ. 2009 Sep 23;86(2):159-62. doi: 10.3354/dao02117.
The Caribbean spiny lobster Panulirus argus is a valuable fishing resource and the trade in frozen lobster tails is an important industry. However, the presence of the pathogenic virus Panulirus argus Virus 1 (PaV1), which causes systemic infection in P. argus and is particularly lethal to juvenile individuals, has not been previously examined in imported/exported lobster products. We used PCR assays to determine the presence of PaV1 in abdominal muscle tissue of 22 frozen P. argus tails exported from Belize to Mexico. Based on their size, the tails belonged to subadult-adult lobsters. Using specific primers targeted for PaV1 resulted in 11 tails showing a specific 499 bp band. The sequence of positive amplified fragments showed a high similarity to PaV1 (95% identity with GenBank accession no. EF206313.1). Although the pathogenicity of PaV1 was not evaluated in the present study, our results provide the first evidence of PaV1 in frozen lobster tails exported in the seafood industry as well as the first molecular evidence of PaV1 in adult lobsters.
加勒比刺龙虾（Panulirus argus）是一种重要的渔业资源，冷冻龙虾尾的贸易是一项重要产业。然而，此前尚未对进出口龙虾产品中是否存在致病性病毒——加勒比刺龙虾病毒1型（PaV1）进行检测，该病毒可在加勒比刺龙虾中引发全身感染，对幼体尤其致命。我们采用聚合酶链反应（PCR）检测法，对从伯利兹出口到墨西哥的22条冷冻加勒比刺龙虾尾的腹肌组织进行检测，以确定其中是否存在PaV1。根据龙虾尾的大小判断，这些龙虾属于亚成体和成体。使用针对PaV1的特异性引物进行检测后，发现有11条龙虾尾呈现出一条499 bp的特异性条带。阳性扩增片段的序列与PaV1具有高度相似性（与GenBank登录号为EF206313.1的序列一致性达95%）。尽管本研究未对PaV1的致病性进行评估，但我们的结果首次证明了海鲜产业出口的冷冻龙虾尾中存在PaV1，同时也是首次在成体龙虾中获得PaV1的分子证据。
