Coschigano P W, Magasanik B
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Mol Cell Biol. 1991 Feb;11(2):822-32. doi: 10.1128/mcb.11.2.822-832.1991.
The URE2 gene of Saccharomyces cerevisiae has been cloned and sequenced. It encodes a predicted polypeptide of 354 amino acids with a molecular weight of 40,226. Deletion of the first 63 amino acids does not have any effect on the function of the protein. Studies with disruption alleles of the URE2 and GLN3 genes showed that both genes regulate GLN1 and GDH2, the structural genes for glutamine synthetase and NAD-linked glutamate dehydrogenase, respectively, at the transcriptional level, but expression of the regulatory genes does not appear to be regulated. Active URE2 gene product was required for the inactivation of glutamine synthetase upon addition of glutamine to cells growing with glutamate as the source of nitrogen. The predicted URE2 gene product has homology to glutathione S-transferases. The gene has been mapped to chromosome XIV, 5.9 map units from petX and 3.4 map units from kex2.
酿酒酵母的URE2基因已被克隆和测序。它编码一个预测的由354个氨基酸组成的多肽，分子量为40226。删除前63个氨基酸对该蛋白质的功能没有任何影响。对URE2和GLN3基因的缺失等位基因的研究表明，这两个基因分别在转录水平上调节谷氨酰胺合成酶和NAD连接的谷氨酸脱氢酶的结构基因GLN1和GDH2，但调节基因的表达似乎不受调控。当向以谷氨酸为氮源生长的细胞中添加谷氨酰胺时，谷氨酰胺合成酶的失活需要活性URE2基因产物。预测的URE2基因产物与谷胱甘肽S-转移酶具有同源性。该基因已被定位到第十四号染色体上，距离petX 5.9个图距单位，距离kex2 3.4个图距单位。
