Orr-Weaver T L, Szostak J W, Rothstein R J
Methods Enzymol. 1983;101:228-45. doi: 10.1016/0076-6879(83)01017-4.
Techniques for high frequency yeast transformation have been described. A double-strand break introduced by restriction enzyme cleavage can be used to direct a plasmid to integrate into a particular chromosomal locus. Plasmids containing a double-strand gap can be used in a straightforward method for the isolation and mapping of chromosomal alleles. These techniques extend the genetic applications of yeast transformation.
高频酵母转化技术已被描述。通过限制酶切割引入的双链断裂可用于引导质粒整合到特定的染色体位点。含有双链缺口的质粒可用于一种直接的方法来分离和定位染色体等位基因。这些技术扩展了酵母转化的遗传应用。
